Enhancement of 1,25-dihydroxyvitamin D3- and all-trans retinoic acid-induced differentiation of human leukemia HL-60 cells by Phyllostachys nigra var. henonis

Seung Hyun Kim, Tae Sung Kim, Hyo Jung Lee, Jin Cheol Yoo

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Human myeloid leukemia HL-60 cells are differentiated into monocytic or granulocytic lineage when treated with 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3] or all-trans retinoic acid (RA), respectively. In this study, the effect of acetone fraction prepared from bamboo leaf on cell differentiation was investigated in a HL-60 cell culture system. Treatment of HL-60 cells with 50-400 μg/ml acetone fraction of bamboo leaf for 72 hr inhibited cell proliferation and induced a little increase in cell differentiation, as demonstrated by the MTT and nitroblue tetrazolium reduction assay. Interestingly, synergistic induction of HL-60 cell differentiation was observed when the acetone fraction of bamboo leaf was combined with either 5 nM 1,25-(OH)2D3 or 50 nM all-trans RA. Flow cytometric analysis indicated that combinations of 1,25-(OH)2D3 and the acetone fraction of bamboo leaf stimulated differentiation predominantly to monocytes, whereas combinations of all-trans RA and the acetone fraction of bamboo leaf stimulated differentiation predominantly to granulocytes. These results suggest that the acetone fraction of bamboo leaf enhanced leukemia cell differentiation and suggest a possibility of bamboo in the treatment of leukemia.

Original languageEnglish
Pages (from-to)119-129
Number of pages11
JournalImmunopharmacology and Immunotoxicology
Volume29
Issue number1
DOIs
Publication statusPublished - 2007 Jan

Keywords

  • Bamboo Leaf
  • Cell Differentiation
  • HL-60 Cell
  • Human Leukemia
  • Phyllostachys Nigra var. Henonis

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Toxicology
  • Pharmacology

Fingerprint Dive into the research topics of 'Enhancement of 1,25-dihydroxyvitamin D3- and all-trans retinoic acid-induced differentiation of human leukemia HL-60 cells by Phyllostachys nigra var. henonis'. Together they form a unique fingerprint.

Cite this