Enhancement of 1,25-dihydroxyvitamin D3-and all-trans retinoic acid-induced HL-60 leukemia cell differentiation by panax ginseng

Seung Hyun Kim, Seung S. Cho, Jaya R. Simkhada, Hyo Jeong Lee, Si Wouk Kim, Tae Sung Kim, Jin Cheol Yoo

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Ginseng (Panax ginseng C.A. Meyer) has a wide range of therapeutic uses including cancer treatment. Human promyelocytic leukemia cells differentiate into monocytes or granulocytes when treated with 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3] or all-trans retinoic acid (ATRA). Treatment of HL-60 cells with zero to 100μg/ml of a methanol extract of ginseng for 72 h induced a small increase in cell differentiation. Surprisingly, a synergistic induction of differentiation was observed when HL-60 cells were treated with ATRA or 1,25-(OH)2D3 and the extract. The inhibitors of protein kinase C (PKC) and extracellular signal-regulated kinase (ERK), but not of phosphoinositide 3-kinase (PI3-K), inhibited the HL-60 differentiation induced by the extract in combination with ATRA or 1,25-(OH)2D3, signifying that PKC and ERK were involved in the cell differentiation enhancement by the extract. These results suggest that the ability of a methanol extract of ginseng to enhance the differentiation potential of ATRA or 1,25-(OH)2D3 may improve the ultimate outcome of acute promyelocytic leukemia therapy.

Original languageEnglish
Pages (from-to)1048-1053
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Volume73
Issue number5
DOIs
Publication statusPublished - 2009 Jun 18

Fingerprint

Panax
Calcitriol
HL-60 Cells
Tretinoin
Cell Differentiation
Leukemia
Extracellular Signal-Regulated MAP Kinases
Protein Kinase C
Methanol
Acute Promyelocytic Leukemia
1-Phosphatidylinositol 4-Kinase
Oncology
Therapeutic Uses
Phosphatidylinositols
Granulocytes
Monocytes
Phosphotransferases
Neoplasms
Therapeutics

Keywords

  • 1,25-dihydroxyvitamin D
  • All-trans retinoic acid
  • HL-60 cells
  • Leukemia
  • Panax ginseng

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Molecular Biology
  • Analytical Chemistry
  • Organic Chemistry
  • Applied Microbiology and Biotechnology

Cite this

Enhancement of 1,25-dihydroxyvitamin D3-and all-trans retinoic acid-induced HL-60 leukemia cell differentiation by panax ginseng. / Kim, Seung Hyun; Cho, Seung S.; Simkhada, Jaya R.; Lee, Hyo Jeong; Kim, Si Wouk; Kim, Tae Sung; Yoo, Jin Cheol.

In: Bioscience, Biotechnology and Biochemistry, Vol. 73, No. 5, 18.06.2009, p. 1048-1053.

Research output: Contribution to journalArticle

Kim, Seung Hyun ; Cho, Seung S. ; Simkhada, Jaya R. ; Lee, Hyo Jeong ; Kim, Si Wouk ; Kim, Tae Sung ; Yoo, Jin Cheol. / Enhancement of 1,25-dihydroxyvitamin D3-and all-trans retinoic acid-induced HL-60 leukemia cell differentiation by panax ginseng. In: Bioscience, Biotechnology and Biochemistry. 2009 ; Vol. 73, No. 5. pp. 1048-1053.
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