Enhancement of 1,25-dihydroxyvitamin D3- and all-trans retinoic acid-induced differentiation of human leukemia HL-60 cells by blood shell, Scapharca broughtonii

Seung Hyun Kim, Tae Sung Kim, June Woo Lee, Jin Cheol Yoo

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Human myeloid leukemia HL-60 cells are differentiated into monocytic or granulocytic lineage when treated with 1,25-dihydroxyvitamin D3 (1,25-[OH]2D3) or all-trans retinoic acid (RA), respectively. In this study, the effect of ethanol fraction prepared from the blood shell, Scapharca broughtonii, on cell differentiation was investigated in an HL-60 cell culture system. Treatment of HL-60 cells with 2.5- to 20-μg/mL ethanol fraction of the blood shell for 72 h inhibited cell proliferation and induced a 20% increase in cell differentiation, as demonstrated by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium and nitroblue tetrazolium reduction assay. Interestingly, synergistic induction of HL-60 cell differentiation was observed when the ethanol fraction of the blood shell was combined with either 5-nM 1,25-(OH)2D3 or 50-nM all-trans RA. Flow cytometric analysis and morphologic studies confirmed that combinations of either 1,25-(OH)2D3 or all-trans RA and the ethanol fraction of the blood ark shell stimulated leukemia cell differentiation. These results suggest a possibility of blood shell in the treatment of human leukemia.

Original languageEnglish
Pages (from-to)96-106
Number of pages11
JournalJournal of Food Biochemistry
Volume32
Issue number1
DOIs
Publication statusPublished - 2008 Feb 1

ASJC Scopus subject areas

  • Food Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

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