Human myeloid leukemia HL-60 cells are differentiated into monocytic or granulocytic lineage when treated with 1,25-dihydroxyvitamin D3 (1,25-[OH]2D3) or all-trans retinoic acid (RA), respectively. In this study, the effect of ethanol fraction prepared from the blood shell, Scapharca broughtonii, on cell differentiation was investigated in an HL-60 cell culture system. Treatment of HL-60 cells with 2.5- to 20-μg/mL ethanol fraction of the blood shell for 72 h inhibited cell proliferation and induced a 20% increase in cell differentiation, as demonstrated by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium and nitroblue tetrazolium reduction assay. Interestingly, synergistic induction of HL-60 cell differentiation was observed when the ethanol fraction of the blood shell was combined with either 5-nM 1,25-(OH)2D3 or 50-nM all-trans RA. Flow cytometric analysis and morphologic studies confirmed that combinations of either 1,25-(OH)2D3 or all-trans RA and the ethanol fraction of the blood ark shell stimulated leukemia cell differentiation. These results suggest a possibility of blood shell in the treatment of human leukemia.
ASJC Scopus subject areas
- Food Science
- Biochemistry, Genetics and Molecular Biology(all)