Enhancement of astaxanthin production using Haematococcus pluvialis with novel LED wavelength shift strategy

Tianqi Xi, Dae Geun Kim, Seong Woon Roh, Jong Soon Choi, Yoon-E Choi

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Haematococcus pluvialis is a green microalga of particular interest, since it is considered the best potential natural source of astaxanthin, which is widely used as an additive for natural pigmentation. In addition, astaxanthin has recently garnered commercial interest as a nutraceutical, cosmetic, and pharmaceutical. However, producing astaxanthin from H. pluvialis necessitates separation with distinctive culture conditions, dividing between the microalgae growth and the astaxanthin production stages. Light-emitting diodes (LEDs) have emerged as a replacement for traditional light sources, and LED applications are now rapidly expanding to multiple areas in fields such as biotechnology. However, further detail application into microalgae biotechnology remains limited. In this study, we have attempted to establish new protocols based on the specific wavelength of LEDs for the cultivation and production of astaxanthin using H. pluvialis. Specifically, we applied red LEDs for microalgae cell growth and then switched to blue LEDs to induce astaxanthin biosynthesis. The result showed that astaxanthin productions based on a wavelength shift from red to blue were significantly increased, compared to those with continuous illumination using red LEDs. Furthermore, additional increase of astaxanthin production was achieved with simultaneous application of exogenous carbon with blue LED illumination. Our approach based on the proper manipulation of LED wavelengths upon H. pluvialis cell stages will enable the improvement of biomass and enhance astaxanthin production using H. pluvialis.

Original languageEnglish
Pages (from-to)6231-6238
Number of pages8
JournalApplied Microbiology and Biotechnology
Volume100
Issue number14
DOIs
Publication statusPublished - 2016 Jul 1

Fingerprint

Light
Microalgae
Biotechnology
Lighting
astaxanthine
Pigmentation
Growth
Dietary Supplements
Cosmetics
Biomass
Carbon
Pharmaceutical Preparations

Keywords

  • Astaxanthin
  • Carbon source
  • Haematococcus pluvialis
  • LED
  • Wavelength shift

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Enhancement of astaxanthin production using Haematococcus pluvialis with novel LED wavelength shift strategy. / Xi, Tianqi; Kim, Dae Geun; Roh, Seong Woon; Choi, Jong Soon; Choi, Yoon-E.

In: Applied Microbiology and Biotechnology, Vol. 100, No. 14, 01.07.2016, p. 6231-6238.

Research output: Contribution to journalArticle

Xi, Tianqi ; Kim, Dae Geun ; Roh, Seong Woon ; Choi, Jong Soon ; Choi, Yoon-E. / Enhancement of astaxanthin production using Haematococcus pluvialis with novel LED wavelength shift strategy. In: Applied Microbiology and Biotechnology. 2016 ; Vol. 100, No. 14. pp. 6231-6238.
@article{e4817853967c40f8a465eecfdec0f720,
title = "Enhancement of astaxanthin production using Haematococcus pluvialis with novel LED wavelength shift strategy",
abstract = "Haematococcus pluvialis is a green microalga of particular interest, since it is considered the best potential natural source of astaxanthin, which is widely used as an additive for natural pigmentation. In addition, astaxanthin has recently garnered commercial interest as a nutraceutical, cosmetic, and pharmaceutical. However, producing astaxanthin from H. pluvialis necessitates separation with distinctive culture conditions, dividing between the microalgae growth and the astaxanthin production stages. Light-emitting diodes (LEDs) have emerged as a replacement for traditional light sources, and LED applications are now rapidly expanding to multiple areas in fields such as biotechnology. However, further detail application into microalgae biotechnology remains limited. In this study, we have attempted to establish new protocols based on the specific wavelength of LEDs for the cultivation and production of astaxanthin using H. pluvialis. Specifically, we applied red LEDs for microalgae cell growth and then switched to blue LEDs to induce astaxanthin biosynthesis. The result showed that astaxanthin productions based on a wavelength shift from red to blue were significantly increased, compared to those with continuous illumination using red LEDs. Furthermore, additional increase of astaxanthin production was achieved with simultaneous application of exogenous carbon with blue LED illumination. Our approach based on the proper manipulation of LED wavelengths upon H. pluvialis cell stages will enable the improvement of biomass and enhance astaxanthin production using H. pluvialis.",
keywords = "Astaxanthin, Carbon source, Haematococcus pluvialis, LED, Wavelength shift",
author = "Tianqi Xi and Kim, {Dae Geun} and Roh, {Seong Woon} and Choi, {Jong Soon} and Yoon-E Choi",
year = "2016",
month = "7",
day = "1",
doi = "10.1007/s00253-016-7301-6",
language = "English",
volume = "100",
pages = "6231--6238",
journal = "Applied Microbiology and Biotechnology",
issn = "0175-7598",
publisher = "Springer Verlag",
number = "14",

}

TY - JOUR

T1 - Enhancement of astaxanthin production using Haematococcus pluvialis with novel LED wavelength shift strategy

AU - Xi, Tianqi

AU - Kim, Dae Geun

AU - Roh, Seong Woon

AU - Choi, Jong Soon

AU - Choi, Yoon-E

PY - 2016/7/1

Y1 - 2016/7/1

N2 - Haematococcus pluvialis is a green microalga of particular interest, since it is considered the best potential natural source of astaxanthin, which is widely used as an additive for natural pigmentation. In addition, astaxanthin has recently garnered commercial interest as a nutraceutical, cosmetic, and pharmaceutical. However, producing astaxanthin from H. pluvialis necessitates separation with distinctive culture conditions, dividing between the microalgae growth and the astaxanthin production stages. Light-emitting diodes (LEDs) have emerged as a replacement for traditional light sources, and LED applications are now rapidly expanding to multiple areas in fields such as biotechnology. However, further detail application into microalgae biotechnology remains limited. In this study, we have attempted to establish new protocols based on the specific wavelength of LEDs for the cultivation and production of astaxanthin using H. pluvialis. Specifically, we applied red LEDs for microalgae cell growth and then switched to blue LEDs to induce astaxanthin biosynthesis. The result showed that astaxanthin productions based on a wavelength shift from red to blue were significantly increased, compared to those with continuous illumination using red LEDs. Furthermore, additional increase of astaxanthin production was achieved with simultaneous application of exogenous carbon with blue LED illumination. Our approach based on the proper manipulation of LED wavelengths upon H. pluvialis cell stages will enable the improvement of biomass and enhance astaxanthin production using H. pluvialis.

AB - Haematococcus pluvialis is a green microalga of particular interest, since it is considered the best potential natural source of astaxanthin, which is widely used as an additive for natural pigmentation. In addition, astaxanthin has recently garnered commercial interest as a nutraceutical, cosmetic, and pharmaceutical. However, producing astaxanthin from H. pluvialis necessitates separation with distinctive culture conditions, dividing between the microalgae growth and the astaxanthin production stages. Light-emitting diodes (LEDs) have emerged as a replacement for traditional light sources, and LED applications are now rapidly expanding to multiple areas in fields such as biotechnology. However, further detail application into microalgae biotechnology remains limited. In this study, we have attempted to establish new protocols based on the specific wavelength of LEDs for the cultivation and production of astaxanthin using H. pluvialis. Specifically, we applied red LEDs for microalgae cell growth and then switched to blue LEDs to induce astaxanthin biosynthesis. The result showed that astaxanthin productions based on a wavelength shift from red to blue were significantly increased, compared to those with continuous illumination using red LEDs. Furthermore, additional increase of astaxanthin production was achieved with simultaneous application of exogenous carbon with blue LED illumination. Our approach based on the proper manipulation of LED wavelengths upon H. pluvialis cell stages will enable the improvement of biomass and enhance astaxanthin production using H. pluvialis.

KW - Astaxanthin

KW - Carbon source

KW - Haematococcus pluvialis

KW - LED

KW - Wavelength shift

UR - http://www.scopus.com/inward/record.url?scp=84957652735&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84957652735&partnerID=8YFLogxK

U2 - 10.1007/s00253-016-7301-6

DO - 10.1007/s00253-016-7301-6

M3 - Article

C2 - 26860938

AN - SCOPUS:84957652735

VL - 100

SP - 6231

EP - 6238

JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

SN - 0175-7598

IS - 14

ER -