Enhancement of biomass production and soluble methane monooxygenase activity in continuous cultures of Methylosinus trichosporium OB3b

Jeewon Lee, Bhupendra K. Soni, Robert L. Kelley

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Using cell recycle, the concentration of M. trichosporium OB3b was increased to more than 12 g/L in continuous culture and biomass productivity was enhanced up to 0.708 g/L/h. The optimal temperature and pH for soluble MMO activity were 35°C and 6.2-6.4. The Optimal concentrations of methanol and sodium formate, as cofactor regenerators, were 0.5 mM and 10 mM, respectively, at which the soluble MMO activity was about 8 times enhanced.

Original languageEnglish
Pages (from-to)897-902
Number of pages6
JournalBiotechnology Letters
Volume18
Issue number8
Publication statusPublished - 1996
Externally publishedYes

Fingerprint

formic acid
methane monooxygenase
Methylosinus trichosporium
Regenerators
Biomass
Methanol
Methane
Productivity
Sodium
Temperature

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Enhancement of biomass production and soluble methane monooxygenase activity in continuous cultures of Methylosinus trichosporium OB3b. / Lee, Jeewon; Soni, Bhupendra K.; Kelley, Robert L.

In: Biotechnology Letters, Vol. 18, No. 8, 1996, p. 897-902.

Research output: Contribution to journalArticle

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