Using cell recycle, the concentration of M. trichosporium OB3b was increased to more than 12 g/L in continuous culture and biomass productivity was enhanced up to 0.708 g/L/h. The optimal temperature and pH for soluble MMO activity were 35°C and 6.2-6.4. The Optimal concentrations of methanol and sodium formate, as cofactor regenerators, were 0.5 mM and 10 mM, respectively, at which the soluble MMO activity was about 8 times enhanced.
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology