Enhancement of bone regeneration using osteogenic-induced adipose- derived stem cells combined with demineralized bone matrix in a rat critically-sized calvarial defect model

Hyun Peel Kim, Yi hwa Ji, Seung Chul Rhee, Eun-Sang Dhong, Seung Ha Park, Eul Sik Yoon

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28 Citations (Scopus)

Abstract

Introduction: Human adipose tissue contains pluripotent stem cells that are similar to bone marrow-derived stem cells. The present study examined whether osteogenic induced adipose-derived stem cells (ASCs) could enhance the osteogenic capacity of demineralized bone matrix and accelerate bone formation in a rat critically-sized calvarial defect model. Materials and Methods: Forty Sprague-Dawley rats were divided randomly into four groups containing 10 rats per each group (Control, 0.05 cc fibrin glue (25 mg/ml) and 0.05 cc thrombin (130 U/ml); DBX, control + 0.2 g DBX ®; ASC, DBX + 1 x 10 5 ASCs/g; iASC, DBX + 1 x 10 5 osteogenic-induced ASCs/g). After osteogenic differentiation of ASCs, alkaline phosphatase and von Kossa staining were performed each week to determine the extent of differentiation and mineralization. An 8-mm critical size circular defect was made in the calvarial bone of each rat. The specimens were harvested 8 weeks after implantation, and radiographic and histological evaluations were carried out. New bone formation was quantified by radiodensitometric analysis of the calvarial sections. Statistical analysis was accomplished using a Mann-Whitney test and Kruskal-Wallis test at a significance level of P<0.05. Results: Alkaline phosphatase and von Kossa staining showed that the osteogenic-induced ASCs yielded higher osteogenic differentiation at 3 weeks. The calvarial defect was filled more in the iASC group compared to the other groups, as demonstrated by the gross appearance of the specimen and radiologic evaluation. The mean radiodensity of the control, DBX, ASC, and iASC group was 16.78%, 39.94%, 25.58%, and 51.31%, respectively, and these were significantly different (P=0.034). Histomorphological evaluation confirmed that new bone formation was accelerated and enhanced by the osteogenic-induced ASCs. Conclusions: ASCs produced greater osteogenic differentiation at 3 weeks. Osteogenic regeneration was accelerated and enhanced in vivo with the osteogenic-induced ASCs, compared to undifferentiated ASCs. Osteogenic-induced ASCs are an excellent and promising candidate for regenerative medicine and tissue engineering application.

Original languageEnglish
Pages (from-to)165-172
Number of pages8
JournalCurrent Stem Cell Research and Therapy
Volume7
Issue number3
DOIs
Publication statusPublished - 2012 May 1

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Bone Matrix
Bone Regeneration
Stem Cells
Osteogenesis
Alkaline Phosphatase
Staining and Labeling
Pluripotent Stem Cells
Fibrin Tissue Adhesive
Regenerative Medicine
Tissue Engineering
Thrombin
Sprague Dawley Rats
Adipose Tissue
Regeneration

Keywords

  • Adipose-derived stem cells
  • Bone regeneration
  • Demineralized bone matrix
  • Fat
  • Mesenchymal stem cells
  • Osteogenic differentiation
  • Rat

ASJC Scopus subject areas

  • Medicine (miscellaneous)

Cite this

@article{834c9218634d44cabce321e87189d9e2,
title = "Enhancement of bone regeneration using osteogenic-induced adipose- derived stem cells combined with demineralized bone matrix in a rat critically-sized calvarial defect model",
abstract = "Introduction: Human adipose tissue contains pluripotent stem cells that are similar to bone marrow-derived stem cells. The present study examined whether osteogenic induced adipose-derived stem cells (ASCs) could enhance the osteogenic capacity of demineralized bone matrix and accelerate bone formation in a rat critically-sized calvarial defect model. Materials and Methods: Forty Sprague-Dawley rats were divided randomly into four groups containing 10 rats per each group (Control, 0.05 cc fibrin glue (25 mg/ml) and 0.05 cc thrombin (130 U/ml); DBX, control + 0.2 g DBX {\circledR}; ASC, DBX + 1 x 10 5 ASCs/g; iASC, DBX + 1 x 10 5 osteogenic-induced ASCs/g). After osteogenic differentiation of ASCs, alkaline phosphatase and von Kossa staining were performed each week to determine the extent of differentiation and mineralization. An 8-mm critical size circular defect was made in the calvarial bone of each rat. The specimens were harvested 8 weeks after implantation, and radiographic and histological evaluations were carried out. New bone formation was quantified by radiodensitometric analysis of the calvarial sections. Statistical analysis was accomplished using a Mann-Whitney test and Kruskal-Wallis test at a significance level of P<0.05. Results: Alkaline phosphatase and von Kossa staining showed that the osteogenic-induced ASCs yielded higher osteogenic differentiation at 3 weeks. The calvarial defect was filled more in the iASC group compared to the other groups, as demonstrated by the gross appearance of the specimen and radiologic evaluation. The mean radiodensity of the control, DBX, ASC, and iASC group was 16.78{\%}, 39.94{\%}, 25.58{\%}, and 51.31{\%}, respectively, and these were significantly different (P=0.034). Histomorphological evaluation confirmed that new bone formation was accelerated and enhanced by the osteogenic-induced ASCs. Conclusions: ASCs produced greater osteogenic differentiation at 3 weeks. Osteogenic regeneration was accelerated and enhanced in vivo with the osteogenic-induced ASCs, compared to undifferentiated ASCs. Osteogenic-induced ASCs are an excellent and promising candidate for regenerative medicine and tissue engineering application.",
keywords = "Adipose-derived stem cells, Bone regeneration, Demineralized bone matrix, Fat, Mesenchymal stem cells, Osteogenic differentiation, Rat",
author = "Kim, {Hyun Peel} and Ji, {Yi hwa} and Rhee, {Seung Chul} and Eun-Sang Dhong and Park, {Seung Ha} and Yoon, {Eul Sik}",
year = "2012",
month = "5",
day = "1",
doi = "10.2174/157488812799859847",
language = "English",
volume = "7",
pages = "165--172",
journal = "Current Stem Cell Research and Therapy",
issn = "1574-888X",
publisher = "Bentham Science Publishers B.V.",
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}

TY - JOUR

T1 - Enhancement of bone regeneration using osteogenic-induced adipose- derived stem cells combined with demineralized bone matrix in a rat critically-sized calvarial defect model

AU - Kim, Hyun Peel

AU - Ji, Yi hwa

AU - Rhee, Seung Chul

AU - Dhong, Eun-Sang

AU - Park, Seung Ha

AU - Yoon, Eul Sik

PY - 2012/5/1

Y1 - 2012/5/1

N2 - Introduction: Human adipose tissue contains pluripotent stem cells that are similar to bone marrow-derived stem cells. The present study examined whether osteogenic induced adipose-derived stem cells (ASCs) could enhance the osteogenic capacity of demineralized bone matrix and accelerate bone formation in a rat critically-sized calvarial defect model. Materials and Methods: Forty Sprague-Dawley rats were divided randomly into four groups containing 10 rats per each group (Control, 0.05 cc fibrin glue (25 mg/ml) and 0.05 cc thrombin (130 U/ml); DBX, control + 0.2 g DBX ®; ASC, DBX + 1 x 10 5 ASCs/g; iASC, DBX + 1 x 10 5 osteogenic-induced ASCs/g). After osteogenic differentiation of ASCs, alkaline phosphatase and von Kossa staining were performed each week to determine the extent of differentiation and mineralization. An 8-mm critical size circular defect was made in the calvarial bone of each rat. The specimens were harvested 8 weeks after implantation, and radiographic and histological evaluations were carried out. New bone formation was quantified by radiodensitometric analysis of the calvarial sections. Statistical analysis was accomplished using a Mann-Whitney test and Kruskal-Wallis test at a significance level of P<0.05. Results: Alkaline phosphatase and von Kossa staining showed that the osteogenic-induced ASCs yielded higher osteogenic differentiation at 3 weeks. The calvarial defect was filled more in the iASC group compared to the other groups, as demonstrated by the gross appearance of the specimen and radiologic evaluation. The mean radiodensity of the control, DBX, ASC, and iASC group was 16.78%, 39.94%, 25.58%, and 51.31%, respectively, and these were significantly different (P=0.034). Histomorphological evaluation confirmed that new bone formation was accelerated and enhanced by the osteogenic-induced ASCs. Conclusions: ASCs produced greater osteogenic differentiation at 3 weeks. Osteogenic regeneration was accelerated and enhanced in vivo with the osteogenic-induced ASCs, compared to undifferentiated ASCs. Osteogenic-induced ASCs are an excellent and promising candidate for regenerative medicine and tissue engineering application.

AB - Introduction: Human adipose tissue contains pluripotent stem cells that are similar to bone marrow-derived stem cells. The present study examined whether osteogenic induced adipose-derived stem cells (ASCs) could enhance the osteogenic capacity of demineralized bone matrix and accelerate bone formation in a rat critically-sized calvarial defect model. Materials and Methods: Forty Sprague-Dawley rats were divided randomly into four groups containing 10 rats per each group (Control, 0.05 cc fibrin glue (25 mg/ml) and 0.05 cc thrombin (130 U/ml); DBX, control + 0.2 g DBX ®; ASC, DBX + 1 x 10 5 ASCs/g; iASC, DBX + 1 x 10 5 osteogenic-induced ASCs/g). After osteogenic differentiation of ASCs, alkaline phosphatase and von Kossa staining were performed each week to determine the extent of differentiation and mineralization. An 8-mm critical size circular defect was made in the calvarial bone of each rat. The specimens were harvested 8 weeks after implantation, and radiographic and histological evaluations were carried out. New bone formation was quantified by radiodensitometric analysis of the calvarial sections. Statistical analysis was accomplished using a Mann-Whitney test and Kruskal-Wallis test at a significance level of P<0.05. Results: Alkaline phosphatase and von Kossa staining showed that the osteogenic-induced ASCs yielded higher osteogenic differentiation at 3 weeks. The calvarial defect was filled more in the iASC group compared to the other groups, as demonstrated by the gross appearance of the specimen and radiologic evaluation. The mean radiodensity of the control, DBX, ASC, and iASC group was 16.78%, 39.94%, 25.58%, and 51.31%, respectively, and these were significantly different (P=0.034). Histomorphological evaluation confirmed that new bone formation was accelerated and enhanced by the osteogenic-induced ASCs. Conclusions: ASCs produced greater osteogenic differentiation at 3 weeks. Osteogenic regeneration was accelerated and enhanced in vivo with the osteogenic-induced ASCs, compared to undifferentiated ASCs. Osteogenic-induced ASCs are an excellent and promising candidate for regenerative medicine and tissue engineering application.

KW - Adipose-derived stem cells

KW - Bone regeneration

KW - Demineralized bone matrix

KW - Fat

KW - Mesenchymal stem cells

KW - Osteogenic differentiation

KW - Rat

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JF - Current Stem Cell Research and Therapy

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