Abstract
Melanoma is a malignant skin cancer that displays a high rate of tumor cell migration and metastasis. This study examined how corticotropin-releasing hormone (CRH) affects the migration of melanoma cells in order to further understand the relationship between stress and tumor cell migration. The migration assay data showed that CRH treatment increased the level of B16F10 cell migration in a dose- and time-dependent manner. To determine whether the extracellular signal-regulated protein kinase 1/2 (ERK1/2) signaling pathway is involved in the upregulation of melanoma migration, cells were pretreated with an inhibitor of ERK1/2 (PD098059). The pretreatment of PD098059 blocked the increase in cell migration. Furthermore, CRH induced the phosphorylation of ERK1/2. The maximum activation of ERK1/2 by CRH was observed at 15min. Taken together, these results suggest that CRH is an important mediator that regulates the migration of melanoma cells in the skin during stress through the ERK1/2 signaling pathway.
| Original language | English |
|---|---|
| Pages (from-to) | 22-27 |
| Number of pages | 6 |
| Journal | Experimental Dermatology |
| Volume | 16 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2007 Jan 1 |
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Keywords
- Corticotropin-releasing hormone
- Extracellularsignal-regulated protein kinase (ERK) 1/2
- Melanoma
- Migration
ASJC Scopus subject areas
- Dermatology
Cite this
Enhancement of cell migration by corticotropin-releasing hormone through ERK1/2 pathway in murine melanoma cell line, B16F10. / Yang, Yoolhee; Park, Hyunjeong; Yang, Young; Kim, Tae Sung; Bang, Sa Ik; Cho, Dae Ho.
In: Experimental Dermatology, Vol. 16, No. 1, 01.01.2007, p. 22-27.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Enhancement of cell migration by corticotropin-releasing hormone through ERK1/2 pathway in murine melanoma cell line, B16F10
AU - Yang, Yoolhee
AU - Park, Hyunjeong
AU - Yang, Young
AU - Kim, Tae Sung
AU - Bang, Sa Ik
AU - Cho, Dae Ho
PY - 2007/1/1
Y1 - 2007/1/1
N2 - Melanoma is a malignant skin cancer that displays a high rate of tumor cell migration and metastasis. This study examined how corticotropin-releasing hormone (CRH) affects the migration of melanoma cells in order to further understand the relationship between stress and tumor cell migration. The migration assay data showed that CRH treatment increased the level of B16F10 cell migration in a dose- and time-dependent manner. To determine whether the extracellular signal-regulated protein kinase 1/2 (ERK1/2) signaling pathway is involved in the upregulation of melanoma migration, cells were pretreated with an inhibitor of ERK1/2 (PD098059). The pretreatment of PD098059 blocked the increase in cell migration. Furthermore, CRH induced the phosphorylation of ERK1/2. The maximum activation of ERK1/2 by CRH was observed at 15min. Taken together, these results suggest that CRH is an important mediator that regulates the migration of melanoma cells in the skin during stress through the ERK1/2 signaling pathway.
AB - Melanoma is a malignant skin cancer that displays a high rate of tumor cell migration and metastasis. This study examined how corticotropin-releasing hormone (CRH) affects the migration of melanoma cells in order to further understand the relationship between stress and tumor cell migration. The migration assay data showed that CRH treatment increased the level of B16F10 cell migration in a dose- and time-dependent manner. To determine whether the extracellular signal-regulated protein kinase 1/2 (ERK1/2) signaling pathway is involved in the upregulation of melanoma migration, cells were pretreated with an inhibitor of ERK1/2 (PD098059). The pretreatment of PD098059 blocked the increase in cell migration. Furthermore, CRH induced the phosphorylation of ERK1/2. The maximum activation of ERK1/2 by CRH was observed at 15min. Taken together, these results suggest that CRH is an important mediator that regulates the migration of melanoma cells in the skin during stress through the ERK1/2 signaling pathway.
KW - Corticotropin-releasing hormone
KW - Extracellularsignal-regulated protein kinase (ERK) 1/2
KW - Melanoma
KW - Migration
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U2 - 10.1111/j.1600-0625.2006.00511.x
DO - 10.1111/j.1600-0625.2006.00511.x
M3 - Article
C2 - 17181633
AN - SCOPUS:33845399691
VL - 16
SP - 22
EP - 27
JO - Experimental Dermatology
JF - Experimental Dermatology
SN - 0906-6705
IS - 1
ER -