Enhancement of cell migration by corticotropin-releasing hormone through ERK1/2 pathway in murine melanoma cell line, B16F10

Yoolhee Yang, Hyunjeong Park, Young Yang, Tae Sung Kim, Sa Ik Bang, Dae Ho Cho

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Melanoma is a malignant skin cancer that displays a high rate of tumor cell migration and metastasis. This study examined how corticotropin-releasing hormone (CRH) affects the migration of melanoma cells in order to further understand the relationship between stress and tumor cell migration. The migration assay data showed that CRH treatment increased the level of B16F10 cell migration in a dose- and time-dependent manner. To determine whether the extracellular signal-regulated protein kinase 1/2 (ERK1/2) signaling pathway is involved in the upregulation of melanoma migration, cells were pretreated with an inhibitor of ERK1/2 (PD098059). The pretreatment of PD098059 blocked the increase in cell migration. Furthermore, CRH induced the phosphorylation of ERK1/2. The maximum activation of ERK1/2 by CRH was observed at 15min. Taken together, these results suggest that CRH is an important mediator that regulates the migration of melanoma cells in the skin during stress through the ERK1/2 signaling pathway.

Original languageEnglish
Pages (from-to)22-27
Number of pages6
JournalExperimental Dermatology
Volume16
Issue number1
DOIs
Publication statusPublished - 2007 Jan 1

    Fingerprint

Keywords

  • Corticotropin-releasing hormone
  • Extracellularsignal-regulated protein kinase (ERK) 1/2
  • Melanoma
  • Migration

ASJC Scopus subject areas

  • Dermatology

Cite this