Enhancement of hydrolysis of Chlorella vulgaris by hydrochloric acid

Charnho Park, Ja Hyun Lee, Xiaoguang Yang, Hah Young Yoo, Ju Hun Lee, Soo Kweon Lee, Seung Wook Kim

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)


Chlorella vulgaris is considered as one of the potential sources of biomass for bio-based products because it consists of large amounts of carbohydrates. In this study, hydrothermal acid hydrolysis with five different acids (hydrochloric acid, nitric acid, peracetic acid, phosphoric acid, and sulfuric acid) was carried out to produce fermentable sugars (glucose, galactose). The hydrothermal acid hydrolysis by hydrochloric acid showed the highest sugar production. C. vulgaris was hydrolyzed with various concentrations of hydrochloric acid [0.5–10 % (w/w)] and microalgal biomass [20–140 g/L (w/v)] at 121 °C for 20 min. Among the concentrations examined, 2 % hydrochloric acid with 100 g/L biomass yielded the highest conversion of carbohydrates (92.5 %) into reducing sugars. The hydrolysate thus produced from C. vulgaris was fermented using the yeast Brettanomyces custersii H1-603 and obtained bioethanol yield of 0.37 g/g of algal sugars.

Original languageEnglish
Pages (from-to)1015-1021
Number of pages7
JournalBioprocess and Biosystems Engineering
Issue number6
Publication statusPublished - 2016 Jun 1


  • Acid hydrolysis
  • Bioethanol
  • Brettanomyces custersii
  • Chlorella vulgaris
  • Fermentable sugars

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering


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