Enhancement of lipid productivity by ethyl methane sulfonate-mediated random mutagenesis and proteomic analysis in Chlamydomonas reinhardtii

Bongsoo Lee, Gang Guk Choi, Yoon-E Choi, Minji Sung, Min S. Park, Ji Won Yang

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Microalgae-derived biomass has been considered as the most promising candidate for next generation biofuel due to its sustainability and biodegradability. In this study, microalgal strain Chlamydmonas reinhardtii was randomly mutagenized by using a chemical mutagen, ethyl methane sulfonate (EMS) to create mutants showing enhanced lipid production. We identified three random mutants that displayed high lipid production in the screening using Nile red staining. Among those, mutant #128 was selected as candidate for further studies. Our flow cytometry and confocal microscopy analysis revealed that mutant #128 contains larger and more abundant lipid bodies than that of wild-type. Moreover, mutant #128 showed 1.4-fold increased fatty acid methyl ester (FAME) content compared to wild-type under nitrogen depleted condition. In addition, mutant #128 grew faster and accumulated more biomass, resulting in high lipid production. 2D gel electrophoresis and MALDI-TOF analysis used for gene targeting revealed that β-subunit of mitochondrial ATP Synthase and two-component response regulator PilR may be involved in enhanced characteristics of mutant #128. These results show the possibilities of EMS mediated random mutagenesis in generation of mutants to produce high amount of lipid as well as further study for molecular mechanism of mutants.

Original languageEnglish
Pages (from-to)1036-1042
Number of pages7
JournalKorean Journal of Chemical Engineering
Volume31
Issue number6
DOIs
Publication statusPublished - 2014 Jan 1
Externally publishedYes

Fingerprint

Mutagenesis
Methane
Lipids
Productivity
Biomass
Mitochondrial Proton-Translocating ATPases
Mutagens
Biofuels
Flow cytometry
Confocal microscopy
Adenosinetriphosphate
Biodegradability
Electrophoresis
Fatty acids
Sustainable development
Esters
Screening
Nitrogen
Fatty Acids
Gels

Keywords

  • Chlamydmonas reinhardtii
  • Ethyl Methane Sulfonate (EMS)
  • Lipid Productivity
  • Proteomics
  • Random Mutagenesis

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

Enhancement of lipid productivity by ethyl methane sulfonate-mediated random mutagenesis and proteomic analysis in Chlamydomonas reinhardtii. / Lee, Bongsoo; Choi, Gang Guk; Choi, Yoon-E; Sung, Minji; Park, Min S.; Yang, Ji Won.

In: Korean Journal of Chemical Engineering, Vol. 31, No. 6, 01.01.2014, p. 1036-1042.

Research output: Contribution to journalArticle

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