Enhancement of mouse pancreatic regeneration and HIT-T15 cell proliferation with rat pancreatic extract

Young Soo Kim, Jae Jeong Lee, Jun Seop Shin, Hyun Jung Kim, Chan Wha Kim

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

In this study, the effects of rat pancreatic extract (RPE) on regeneration of impaired mouse pancreas and proliferation of β-cell line (HIT-T15) were investigated. RPE from the regenerating pancreas (2 days after 60% pancreatectomy) was treated to cure streptozotocin (STZ) induced diabetes in BALB/c mice. RPE-treated BALB/c mice for 21 consecutive days became euglycemic by day 30 and remained normoglycemic during a 150 day follow-up. Saline treated mice remained hyperglycemic sustained uncontrolled hyperglycemia. Islet neogenesis was observed in RPE-treated mice and confirmed by use of immunocytochemistry. Morphometric analysis of pancreas in reverted RPE-treated mice showed a new population of small islets compared with saline controls and an increased islet number. When HIT-T15 cells were treated with RPE, HIT-T15 cell proliferation and insulin secretion increased with increases in the RPE concentration. These results imply that RPE have the regeneration factors and help in the cure of diabetes.

Original languageEnglish
Pages (from-to)528-532
Number of pages5
JournalBiochemical and biophysical research communications
Volume309
Issue number3
DOIs
Publication statusPublished - 2003 Sep 26

Keywords

  • Blood glucose
  • Diabetes
  • Islets
  • Mitogenic effect
  • Pancreatectomy
  • Rat pancreatic extract

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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