Enhancement of porcine natural killer cell activity by recombinant human and murine IL-12

Dae Ho Cho, Wang J. Lee, Patrick J. Halloran, Giorgio Trinchieri, Yoon B. Kim

Research output: Contribution to journalArticle

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Abstract

These studies describe the effects of recombinant human (rHu) and recombinant murine (rMu) interleukin 12 (IL-12) in the porcine system. To examine the effects of rHu and rMu IL-12, porcine PBL were cultured with rHu and rMu IL-12, and then used for a NK assay. Significant enhancement of porcine NK cell cytotoxicity was observed, and maximal enhancement was reached after 26 hr culture in nanogram quantities (5 ng/ml = 10 units/ml) of rHu and rMu IL-12. MonoclonaI antibody (mAb) specific for human IL-12, C8.6, blocked rHu IL-12 enhanced porcine natural killer (NK) cytotoxicity. It was also shown that rHu IL-12 had additive effects on enhancement of NK cell cytotoxicity with PNK-E/G7 mAbs which react with cytolytic trigger molecules on porcine NK cells. By using purified NK cells, porcine tumor necrosis factor-α (TNF-α) secretion was measured on rHu IL-12 and PNK-E/G7 mAb-activated NK cells. rHu IL-12 and PNK-E/G7 mAbs synergize to induce TNF-α secretion. These data indicate that rHu and rMu IL-12 are cross-reactive in the porcine system and suggest the potential use of the porcine system as an important animal model for further study of rHu IL-12.

Original languageEnglish
Pages (from-to)29-34
Number of pages6
JournalCellular Immunology
Volume172
Issue number1
DOIs
Publication statusPublished - 1996 Aug 25
Externally publishedYes

Fingerprint

Interleukin-12
Human Activities
Natural Killer Cells
Swine
Tumor Necrosis Factor-alpha
Antibodies
Animal Models

ASJC Scopus subject areas

  • Immunology

Cite this

Enhancement of porcine natural killer cell activity by recombinant human and murine IL-12. / Cho, Dae Ho; Lee, Wang J.; Halloran, Patrick J.; Trinchieri, Giorgio; Kim, Yoon B.

In: Cellular Immunology, Vol. 172, No. 1, 25.08.1996, p. 29-34.

Research output: Contribution to journalArticle

Cho, Dae Ho ; Lee, Wang J. ; Halloran, Patrick J. ; Trinchieri, Giorgio ; Kim, Yoon B. / Enhancement of porcine natural killer cell activity by recombinant human and murine IL-12. In: Cellular Immunology. 1996 ; Vol. 172, No. 1. pp. 29-34.
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