Enhancement of sensitivity in interferometric biosensing by using a new biolinker and prebinding antibody

Jae Sook Park, Sung Hyuk Lim, Sang Jun Sim, Heeyeop Chae, Hyun C. Yoon, Sang Sik Yang, Byung Woo Kim

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Recombinant E. coli ACV 1003 (recA::lacZ) was used to measure low concentrations of DNA-darnaging chemicals, which produce β-galactosidase via an SOS regulon system. Very low β-galactosidase activities of less than 0.01 unit/ml, β-galactosidase produced through an SOS response corresponding to the 10 ng/ml (ppb) of DNA damaging chemicals in the environment, can be rapidly determined by using an alternative interferometric biosensor with optically flat thin films of porous silicon rather than by the conventional time-consuming Miller's enzyme assay as well as the ELISA method. In order to enhance the sensitivity in the interferometry, it needs to obtain more uniform distribution and higher biolinking efficiency, whereas interferometric sensing is rapid, cheap, and advantageous in high throughput by using a multiple-well-type chip. In this study, pore size adjusted to 60 rim for the target enzyme β-galactosidase to be bound on both walls of a Si pore and a calyx crown derivative was apllied as a more efficient biolinker. Furthermore, anti-β-galactosidase was previously functionalized with the biolinker for the target β-galactosidase to be specifically bound. When anti-β-galactosidase was bound to the calyx-crown derivative-linked surface, the effective optical thickness was found to be three times as high as that obtained without using anti-β-galactosidase. The resolution obtained was very similar to that afforded by the time-consuming ELISA method; however, the reproducibility was still unsatisfactory, below 1 unit β-galactosidase/ ml, owing to the microscopic non-uniform distribution of the pores in the etched silicon surface.

Original languageEnglish
Pages (from-to)1968-1976
Number of pages9
JournalJournal of Microbiology and Biotechnology
Volume16
Issue number12
Publication statusPublished - 2006 Dec 1
Externally publishedYes

Fingerprint

Galactosidases
Antibodies
Silicon
Crowns
DNA
Enzymes
Enzyme-Linked Immunosorbent Assay
Interferometry
Derivatives
Regulon
Porous silicon
Enzyme Assays
Biosensing Techniques
Biosensors
Escherichia coli
Pore size
Assays
Throughput

Keywords

  • β-galactosidase
  • DNA damaging chemicals
  • Fabry-Ferot fringe
  • Functionalization
  • Si-based biosensor

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Microbiology
  • Bioengineering

Cite this

Enhancement of sensitivity in interferometric biosensing by using a new biolinker and prebinding antibody. / Park, Jae Sook; Lim, Sung Hyuk; Sim, Sang Jun; Chae, Heeyeop; Yoon, Hyun C.; Yang, Sang Sik; Kim, Byung Woo.

In: Journal of Microbiology and Biotechnology, Vol. 16, No. 12, 01.12.2006, p. 1968-1976.

Research output: Contribution to journalArticle

Park, Jae Sook ; Lim, Sung Hyuk ; Sim, Sang Jun ; Chae, Heeyeop ; Yoon, Hyun C. ; Yang, Sang Sik ; Kim, Byung Woo. / Enhancement of sensitivity in interferometric biosensing by using a new biolinker and prebinding antibody. In: Journal of Microbiology and Biotechnology. 2006 ; Vol. 16, No. 12. pp. 1968-1976.
@article{4ff85db9caa64047b175cd1de3aac361,
title = "Enhancement of sensitivity in interferometric biosensing by using a new biolinker and prebinding antibody",
abstract = "Recombinant E. coli ACV 1003 (recA::lacZ) was used to measure low concentrations of DNA-darnaging chemicals, which produce β-galactosidase via an SOS regulon system. Very low β-galactosidase activities of less than 0.01 unit/ml, β-galactosidase produced through an SOS response corresponding to the 10 ng/ml (ppb) of DNA damaging chemicals in the environment, can be rapidly determined by using an alternative interferometric biosensor with optically flat thin films of porous silicon rather than by the conventional time-consuming Miller's enzyme assay as well as the ELISA method. In order to enhance the sensitivity in the interferometry, it needs to obtain more uniform distribution and higher biolinking efficiency, whereas interferometric sensing is rapid, cheap, and advantageous in high throughput by using a multiple-well-type chip. In this study, pore size adjusted to 60 rim for the target enzyme β-galactosidase to be bound on both walls of a Si pore and a calyx crown derivative was apllied as a more efficient biolinker. Furthermore, anti-β-galactosidase was previously functionalized with the biolinker for the target β-galactosidase to be specifically bound. When anti-β-galactosidase was bound to the calyx-crown derivative-linked surface, the effective optical thickness was found to be three times as high as that obtained without using anti-β-galactosidase. The resolution obtained was very similar to that afforded by the time-consuming ELISA method; however, the reproducibility was still unsatisfactory, below 1 unit β-galactosidase/ ml, owing to the microscopic non-uniform distribution of the pores in the etched silicon surface.",
keywords = "β-galactosidase, DNA damaging chemicals, Fabry-Ferot fringe, Functionalization, Si-based biosensor",
author = "Park, {Jae Sook} and Lim, {Sung Hyuk} and Sim, {Sang Jun} and Heeyeop Chae and Yoon, {Hyun C.} and Yang, {Sang Sik} and Kim, {Byung Woo}",
year = "2006",
month = "12",
day = "1",
language = "English",
volume = "16",
pages = "1968--1976",
journal = "Journal of Microbiology and Biotechnology",
issn = "1017-7825",
publisher = "Korean Society for Microbiolog and Biotechnology",
number = "12",

}

TY - JOUR

T1 - Enhancement of sensitivity in interferometric biosensing by using a new biolinker and prebinding antibody

AU - Park, Jae Sook

AU - Lim, Sung Hyuk

AU - Sim, Sang Jun

AU - Chae, Heeyeop

AU - Yoon, Hyun C.

AU - Yang, Sang Sik

AU - Kim, Byung Woo

PY - 2006/12/1

Y1 - 2006/12/1

N2 - Recombinant E. coli ACV 1003 (recA::lacZ) was used to measure low concentrations of DNA-darnaging chemicals, which produce β-galactosidase via an SOS regulon system. Very low β-galactosidase activities of less than 0.01 unit/ml, β-galactosidase produced through an SOS response corresponding to the 10 ng/ml (ppb) of DNA damaging chemicals in the environment, can be rapidly determined by using an alternative interferometric biosensor with optically flat thin films of porous silicon rather than by the conventional time-consuming Miller's enzyme assay as well as the ELISA method. In order to enhance the sensitivity in the interferometry, it needs to obtain more uniform distribution and higher biolinking efficiency, whereas interferometric sensing is rapid, cheap, and advantageous in high throughput by using a multiple-well-type chip. In this study, pore size adjusted to 60 rim for the target enzyme β-galactosidase to be bound on both walls of a Si pore and a calyx crown derivative was apllied as a more efficient biolinker. Furthermore, anti-β-galactosidase was previously functionalized with the biolinker for the target β-galactosidase to be specifically bound. When anti-β-galactosidase was bound to the calyx-crown derivative-linked surface, the effective optical thickness was found to be three times as high as that obtained without using anti-β-galactosidase. The resolution obtained was very similar to that afforded by the time-consuming ELISA method; however, the reproducibility was still unsatisfactory, below 1 unit β-galactosidase/ ml, owing to the microscopic non-uniform distribution of the pores in the etched silicon surface.

AB - Recombinant E. coli ACV 1003 (recA::lacZ) was used to measure low concentrations of DNA-darnaging chemicals, which produce β-galactosidase via an SOS regulon system. Very low β-galactosidase activities of less than 0.01 unit/ml, β-galactosidase produced through an SOS response corresponding to the 10 ng/ml (ppb) of DNA damaging chemicals in the environment, can be rapidly determined by using an alternative interferometric biosensor with optically flat thin films of porous silicon rather than by the conventional time-consuming Miller's enzyme assay as well as the ELISA method. In order to enhance the sensitivity in the interferometry, it needs to obtain more uniform distribution and higher biolinking efficiency, whereas interferometric sensing is rapid, cheap, and advantageous in high throughput by using a multiple-well-type chip. In this study, pore size adjusted to 60 rim for the target enzyme β-galactosidase to be bound on both walls of a Si pore and a calyx crown derivative was apllied as a more efficient biolinker. Furthermore, anti-β-galactosidase was previously functionalized with the biolinker for the target β-galactosidase to be specifically bound. When anti-β-galactosidase was bound to the calyx-crown derivative-linked surface, the effective optical thickness was found to be three times as high as that obtained without using anti-β-galactosidase. The resolution obtained was very similar to that afforded by the time-consuming ELISA method; however, the reproducibility was still unsatisfactory, below 1 unit β-galactosidase/ ml, owing to the microscopic non-uniform distribution of the pores in the etched silicon surface.

KW - β-galactosidase

KW - DNA damaging chemicals

KW - Fabry-Ferot fringe

KW - Functionalization

KW - Si-based biosensor

UR - http://www.scopus.com/inward/record.url?scp=33846533568&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33846533568&partnerID=8YFLogxK

M3 - Article

VL - 16

SP - 1968

EP - 1976

JO - Journal of Microbiology and Biotechnology

JF - Journal of Microbiology and Biotechnology

SN - 1017-7825

IS - 12

ER -