Enhancement of Toll-like receptor 2-mediated immune responses by AIMP1, a novel cytokine, in mouse dendritic cells

Eugene Kim, Hye Jin Hong, Dae Ho Cho, Jung Min Han, Sunghoon Kim, Tae Sung Kim

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Summary: Aminoacyl tRNA synthetase-interacting protein 1 (AIMP1) is a novel pleiotropic cytokine that was identified initially from Meth A-induced fibrosarcoma. It is expressed in the salivary glands, small intestine and large intestine, and is associated with the innate immune system. Previously, we demonstrated that AIMP1 might function as a regulator of innate immune responses by inducing the maturation and activation of bone-marrow-derived dendritic cells (BM-DCs). Toll-like receptors (TLRs) are major pathogen-recognition receptors that are constitutively expressed on DCs. In this study, we attempted to determine whether AIMP1 is capable of regulating the expression of TLRs, and also capable of affecting the TLR-mediated activation of DCs. Expression of TLR1, -2, -3 and -7 was highly induced by AIMP1 treatment in BM-DCs, whereas the expression of other TLRs was either down-regulated or remained unchanged. In particular, the expression of the TLR2 protein was up-regulated by AIMP1 in a time-dependent and dose-dependent manner, and was suppressed upon the addition of BAY11-7082, an inhibitor of nuclear factor-κB. AIMP1 was also shown to increase nuclear factor-κB binding activity. Importantly, AIMP1 enhanced the production of interleukin-6 and interleukin-12, and the expression of co-stimulatory molecules on BM-DCs when combined with lipoteichoic acid or Pam3Cys, two well-known TLR2 agonists. Collectively, these results demonstrate that the AIMP1 protein enhances TLR2-mediated immune responses via the up-regulation of TLR2 expression.

Original languageEnglish
Pages (from-to)73-81
Number of pages9
JournalImmunology
Volume134
Issue number1
DOIs
Publication statusPublished - 2011 Sep 1

Fingerprint

Toll-Like Receptor 2
Toll-Like Receptors
Dendritic Cells
Cytokines
Bone Marrow
Amino Acyl-tRNA Synthetases
Proteins
Fibrosarcoma
Large Intestine
Interleukin-12
Salivary Glands
Innate Immunity
Small Intestine
Immune System
Interleukin-6
Up-Regulation

Keywords

  • Aminoacyl tRNA synthetase-interacting protein 1
  • Cytokines
  • Dendritic cells
  • Rodent
  • Toll-like receptor 2
  • Toll-like receptor 2 agonist

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Enhancement of Toll-like receptor 2-mediated immune responses by AIMP1, a novel cytokine, in mouse dendritic cells. / Kim, Eugene; Hong, Hye Jin; Cho, Dae Ho; Han, Jung Min; Kim, Sunghoon; Kim, Tae Sung.

In: Immunology, Vol. 134, No. 1, 01.09.2011, p. 73-81.

Research output: Contribution to journalArticle

Kim, Eugene ; Hong, Hye Jin ; Cho, Dae Ho ; Han, Jung Min ; Kim, Sunghoon ; Kim, Tae Sung. / Enhancement of Toll-like receptor 2-mediated immune responses by AIMP1, a novel cytokine, in mouse dendritic cells. In: Immunology. 2011 ; Vol. 134, No. 1. pp. 73-81.
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