Enhancement of vaccinia vaccine potency by linkage of tumor antigen gene to gene encoding calreticulin

Chia Jung Hsieh, Tae Woo Kim, Chien Fu Hung, Jeremy Juang, Michelle Moniz, David A K Boyd, Liangmei He, Pei Jer Chen, Chien Hung Chen, T. C. Wu

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Vaccinia vaccines have become important vectors for antigen-specific immunotherapy. Calreticulin has been shown to enhance MHC class I presentation of linked peptide/protein and may be useful for antigen-specific cancer treatment. An innovative vaccine administering antigen linked to calreticulin via a vaccinia vector may generate a potent antigen-specific antitumor response. We tested the efficacy of linking calreticulin (CRT) to model antigen human papilloma virus type 16 (HPV-16) E7 in the context of a vaccinia vaccine (Vac-CRT/E7). Intraperitoneal vaccination of C57BL/6 mice with Vac-CRT/E7 led to a dramatic increase in E7-specific IFN-γ-secreting CD8+ T cells and a potent antitumor effect against E7-expressing tumors compared to immunization with Vac-E7 or Vac-CRT. When compared to other chimeric vaccinia vaccines employing various intracellular targeting strategies previously developed in our lab, Vac-CRT/E7 elicited the highest number of E7-specific CD8+ T cells. Thus, vaccination with vaccinia expressing CRT linked to a tumor antigen may represent an advantageous strategy for cancer immunotherapy.

Original languageEnglish
Pages (from-to)3993-4001
Number of pages9
JournalVaccine
Volume22
Issue number29-30
DOIs
Publication statusPublished - 2004 Sep 28
Externally publishedYes

    Fingerprint

Keywords

  • Calreticulin
  • Cancer immunotherapy
  • Vaccinia vaccines

ASJC Scopus subject areas

  • Medicine(all)
  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

Cite this

Hsieh, C. J., Kim, T. W., Hung, C. F., Juang, J., Moniz, M., Boyd, D. A. K., He, L., Chen, P. J., Chen, C. H., & Wu, T. C. (2004). Enhancement of vaccinia vaccine potency by linkage of tumor antigen gene to gene encoding calreticulin. Vaccine, 22(29-30), 3993-4001. https://doi.org/10.1016/j.vaccine.2004.03.057