Enhancing Fatty Acid Production of Saccharomyces cerevisiae as an Animal Feed Supplement

Seung Kyou You, Young Chul Joo, Dae Hee Kang, Sang Kyu Shin, Jeong Eun Hyeon, Han Min Woo, Youngsoon Um, Chulhwan Park, Sung Ok Han

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5 Citations (Scopus)


Saccharomyces cerevisiae is used for edible purposes, such as human food or as an animal feed supplement. Fatty acids are also beneficial as feed supplements, but S. cerevisiae produces small amounts of fatty acids. In this study, we enhanced fatty acid production of S. cerevisiae by overexpressing acetyl-CoA carboxylase, thioesterase, and malic enzyme associated with fatty acid metabolism. The enhanced strain pAMT showed 2.4-fold higher fatty acids than the wild-type strain. To further increase the fatty acids, various nitrogen sources were analyzed and calcium nitrate was selected as an optimal nitrogen source for fatty acid production. By concentration optimization, 672 mg/L of fatty acids was produced, which was 4.7-fold higher than wild-type strain. These results complement the low level fatty acid production and make it possible to obtain the benefits of fatty acids as an animal feed supplement while, simultaneously, maintaining the advantages of S. cerevisiae.

Original languageEnglish
Pages (from-to)11029-11035
Number of pages7
JournalJournal of Agricultural and Food Chemistry
Issue number50
Publication statusPublished - 2017 Dec 20



  • animal feed supplement
  • cofactor balancing
  • fatty acids production
  • Saccharomyces cerevisiae, nitrogen optimization

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

Cite this

You, S. K., Joo, Y. C., Kang, D. H., Shin, S. K., Hyeon, J. E., Woo, H. M., Um, Y., Park, C., & Han, S. O. (2017). Enhancing Fatty Acid Production of Saccharomyces cerevisiae as an Animal Feed Supplement. Journal of Agricultural and Food Chemistry, 65(50), 11029-11035. https://doi.org/10.1021/acs.jafc.7b04485