Enhancing Proliferation and ECM Expression of Human ACL Fibroblasts by Sonic Vibration

Yuan Yuan Jiang, Jung Keug Park, Hee Hoon Yoon, Hynjin Choi, Chan Wha Kim, Young Kwon Seo

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)


Effects of mechanical vibration on cell activity and behavior remain controversial: There has been evidence on both positive and negative effects. Furthermore, research on the anterior cruciate ligament (ACL) has as yet been limited and the frequency-related effects remain unknown, even though ACL injury is common and an injuredACL hardly spontaneously recovers. The object of this work was to address the influence of mechanical vibration on ACL fibroblasts, to determine the effects of frequencies, and to further study this effect at the cellular level. We found that sonic vibration affectedACL fibroblasts' proliferation and metabolism in a frequency-dependent manner, and 20 Hz gave rise to the most ACL cell activity and comprehensively increased extracellular matrix (ECM) contents, including collagen type I, collagen type III, fibronectin, elastin, tenascin, glycosaminoglycan (GAG), and the cytoskeleton protein vimentin. Thus, our results indicate that sonic vibration possesses frequency-dependent effects on proliferation and productivity of ACL fibroblast with an optimal frequency of 20 Hz under the present stimulation conditions, providing further information for future research in how vibrational stimulation manipulates ACL cellular behavior.

Original languageEnglish
Pages (from-to)476-490
Number of pages15
JournalPreparative Biochemistry and Biotechnology
Issue number5
Publication statusPublished - 2015 Jul 4


  • Extracellular matrix
  • human anterior cruciate ligament fibroblast
  • proliferation
  • sonic vibration

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry


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