Enhancing surface functionality of reduced graphene oxide biosensors by oxygen plasma treatment for Alzheimer's disease diagnosis

Myung Sic Chae, Jinsik Kim, Dahye Jeong, Young Soo Kim, Jee Hoon Roh, Sung Min Lee, Youhee Heo, Ji Yoon Kang, Jeong Hoon Lee, Dae Sung Yoon, Tae Geun Kim, Suk Tai Chang, Kyo Seon Hwang

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

We performed oxygen plasma treatment on reduced graphene oxide (rGO) to improve its surface reactivity with respect to biomolecular interactions. Oxygen-plasma-treated rGO surfaces were employed as reactive interfaces for the detection of amyloid-beta (Aβ) peptides, the pathological hallmarks of Alzheimer's disease (AD), as the target analytes. By measuring the changes in electrical characteristics and confirmation through topographic analysis, the oxygen-plasma-treated rGO sensors had enhanced surface functionality for better antibody immobilization and sensing performance, with a 3.33-fold steeper slope for the electrical responses versus analyte concentration curve (logarithmic scale) compared to the untreated. The elicited biomolecular reactivity of the rGO surfaces with the oxygen plasma treatment remained at 46-51% of the initial value even after aging for 6. h in ambient conditions. This phenomenon was also confirmed by pretreating the rGO surfaces with a blocking agent and subsequently subjecting them to antibody immobilization. Finally, the feasibility of the oxygen-plasma-treated rGO sensors as a diagnostic tool was evaluated with clinical samples of neural-derived exosomal Aβ peptides extracted from apparent AD patients and normal controls (NC). In contrast to the untreated sensors (p=0.0460), the oxygen-plasma-treated rGO sensors showed a significant p-value in the identification of clinical samples of AD and NC subjects (p<0.001). These results suggest that oxygen plasma treatment improves sensor performance without complicated fabrication procedures and should aid in the development of novel diagnostic tools based on carbon nanomaterials.

Original languageEnglish
JournalBiosensors and Bioelectronics
DOIs
Publication statusAccepted/In press - 2016 Aug 25

Keywords

  • Alzheimer's diseases
  • Biosensor
  • Exosome
  • Oxygen plasma treatment
  • Reduced graphene oxide

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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  • Cite this

    Chae, M. S., Kim, J., Jeong, D., Kim, Y. S., Roh, J. H., Lee, S. M., Heo, Y., Kang, J. Y., Lee, J. H., Yoon, D. S., Kim, T. G., Chang, S. T., & Hwang, K. S. (Accepted/In press). Enhancing surface functionality of reduced graphene oxide biosensors by oxygen plasma treatment for Alzheimer's disease diagnosis. Biosensors and Bioelectronics. https://doi.org/10.1016/j.bios.2016.10.049