Abstract
Optimization of the dilution rates has been studied to provide an enhanced sensitivity to toxicity by several recombinant bioluminescent Escherichia coli strains, TV1061 (grpE::luxCDABE), DPD2794 (recA::luxCDABE) and DPD2540 (fabA::luxCDABE), in the two-stage continuous toxicity monitoring system. It was found that the sensitivity of both TV1061 and DPD2794 to a pulse injection of phenol and mitomycin C increased with a decrease in the dilution rate. The sensitivity, however, for all the strains to step injections of the toxic chemicals was found to increase with an increase in the dilution rate up to a certain dilution rate and then decreased, mainly due to the rapid washing out of the injected chemicals. The response kinetics of the strains were explained by evaluating the mode of action of the recombinant bioluminescent bacteria to toxicity with the dilution rate, the operating parameter of minibioreactors under consideration in this study.
| Original language | English |
|---|---|
| Pages (from-to) | 283-288 |
| Number of pages | 6 |
| Journal | Journal of Biotechnology |
| Volume | 93 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2002 Feb 28 |
| Externally published | Yes |
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Keywords
- Dilution rate
- Recombinant bioluminescent bacteria
- Sensitivity
- Toxicity
- Whole cell biosensor
ASJC Scopus subject areas
- Biotechnology
Cite this
Enhancing the sensitivity of a two-stage continuous toxicity monitoring system through the manipulation of the dilution rate. / Gu, Man Bock; Gil, Geun Cheol; Kim, Joong Hyun.
In: Journal of Biotechnology, Vol. 93, No. 3, 28.02.2002, p. 283-288.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Enhancing the sensitivity of a two-stage continuous toxicity monitoring system through the manipulation of the dilution rate
AU - Gu, Man Bock
AU - Gil, Geun Cheol
AU - Kim, Joong Hyun
PY - 2002/2/28
Y1 - 2002/2/28
N2 - Optimization of the dilution rates has been studied to provide an enhanced sensitivity to toxicity by several recombinant bioluminescent Escherichia coli strains, TV1061 (grpE::luxCDABE), DPD2794 (recA::luxCDABE) and DPD2540 (fabA::luxCDABE), in the two-stage continuous toxicity monitoring system. It was found that the sensitivity of both TV1061 and DPD2794 to a pulse injection of phenol and mitomycin C increased with a decrease in the dilution rate. The sensitivity, however, for all the strains to step injections of the toxic chemicals was found to increase with an increase in the dilution rate up to a certain dilution rate and then decreased, mainly due to the rapid washing out of the injected chemicals. The response kinetics of the strains were explained by evaluating the mode of action of the recombinant bioluminescent bacteria to toxicity with the dilution rate, the operating parameter of minibioreactors under consideration in this study.
AB - Optimization of the dilution rates has been studied to provide an enhanced sensitivity to toxicity by several recombinant bioluminescent Escherichia coli strains, TV1061 (grpE::luxCDABE), DPD2794 (recA::luxCDABE) and DPD2540 (fabA::luxCDABE), in the two-stage continuous toxicity monitoring system. It was found that the sensitivity of both TV1061 and DPD2794 to a pulse injection of phenol and mitomycin C increased with a decrease in the dilution rate. The sensitivity, however, for all the strains to step injections of the toxic chemicals was found to increase with an increase in the dilution rate up to a certain dilution rate and then decreased, mainly due to the rapid washing out of the injected chemicals. The response kinetics of the strains were explained by evaluating the mode of action of the recombinant bioluminescent bacteria to toxicity with the dilution rate, the operating parameter of minibioreactors under consideration in this study.
KW - Dilution rate
KW - Recombinant bioluminescent bacteria
KW - Sensitivity
KW - Toxicity
KW - Whole cell biosensor
UR - http://www.scopus.com/inward/record.url?scp=0037186275&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037186275&partnerID=8YFLogxK
U2 - 10.1016/S0168-1656(01)00410-2
DO - 10.1016/S0168-1656(01)00410-2
M3 - Article
C2 - 11755991
AN - SCOPUS:0037186275
VL - 93
SP - 283
EP - 288
JO - Journal of Biotechnology
JF - Journal of Biotechnology
SN - 0168-1656
IS - 3
ER -