Enrichment of Clostridia during the operation of an external-powered bio-electrochemical denitrification system

Sang Hoon Lee; Sanath Kondaveeti; Booki Min; Hee Deung Park

doi:10.1016/j.procbio.2012.11.020

Enrichment of Clostridia during the operation of an external-powered bio-electrochemical denitrification system

Sang Hoon Lee, Sanath Kondaveeti, Booki Min, Hee Deung Park

School of Civil, Environmental and Architectural Engineering

Research output: Contribution to journal › Article › peer-review

57 Citations (Scopus)

Abstract

Bacterial community composition was investigated in a biocathode of an external-powered bio-electrochemical denitrifying system (BEDS), by analyzing 16S rRNA gene sequences determined from a high-throughput 454 pyrosequencing technology. The bacterial community of the inoculum sludge was mainly composed of Proteobacteria, Bacteroidetes, and Acidobacteria. Over 45 days of the BEDS, Bacteroidetes and Firmicutes were enriched on the biocathode. Putative denitrifying Clostridia belonging to the Firmicutes were distinctively detected on the biocathode via phylogenetic analysis and appeared to be ubiquitous in various autotrophic, heterotrophic, and BEDSs. Elucidation of the bacterial community composition in the denitrification system deepens our knowledge about the bacteria involved in bio-electrochemical denitrification.

Original language	English
Pages (from-to)	306-311
Number of pages	6
Journal	Process Biochemistry
Volume	48
Issue number	2
DOIs	https://doi.org/10.1016/j.procbio.2012.11.020
Publication status	Published - 2013 Feb

Keywords

Bio-electrochemical denitrification
Clostridia
Denitrification
Firmicutes
Microbial fuel cell

ASJC Scopus subject areas

Bioengineering
Biochemistry
Applied Microbiology and Biotechnology

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10.1016/j.procbio.2012.11.020

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title = "Enrichment of Clostridia during the operation of an external-powered bio-electrochemical denitrification system",

abstract = "Bacterial community composition was investigated in a biocathode of an external-powered bio-electrochemical denitrifying system (BEDS), by analyzing 16S rRNA gene sequences determined from a high-throughput 454 pyrosequencing technology. The bacterial community of the inoculum sludge was mainly composed of Proteobacteria, Bacteroidetes, and Acidobacteria. Over 45 days of the BEDS, Bacteroidetes and Firmicutes were enriched on the biocathode. Putative denitrifying Clostridia belonging to the Firmicutes were distinctively detected on the biocathode via phylogenetic analysis and appeared to be ubiquitous in various autotrophic, heterotrophic, and BEDSs. Elucidation of the bacterial community composition in the denitrification system deepens our knowledge about the bacteria involved in bio-electrochemical denitrification.",

keywords = "Bio-electrochemical denitrification, Clostridia, Denitrification, Firmicutes, Microbial fuel cell",

author = "Lee, {Sang Hoon} and Sanath Kondaveeti and Booki Min and Park, {Hee Deung}",

note = "Funding Information: This study was funded by a Korea University Grant (to H.-D. Park), a National Research Foundation of Korea Grant (No. 2010-0003940 ) (to B. Min), and a Korea Ministry of Environment (MOE) Grant “Human resource development Project for Energy from Waste & Recycling” (to B. Min).",

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doi = "10.1016/j.procbio.2012.11.020",

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AU - Min, Booki

AU - Park, Hee Deung

N1 - Funding Information: This study was funded by a Korea University Grant (to H.-D. Park), a National Research Foundation of Korea Grant (No. 2010-0003940 ) (to B. Min), and a Korea Ministry of Environment (MOE) Grant “Human resource development Project for Energy from Waste & Recycling” (to B. Min).

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Y1 - 2013/2

N2 - Bacterial community composition was investigated in a biocathode of an external-powered bio-electrochemical denitrifying system (BEDS), by analyzing 16S rRNA gene sequences determined from a high-throughput 454 pyrosequencing technology. The bacterial community of the inoculum sludge was mainly composed of Proteobacteria, Bacteroidetes, and Acidobacteria. Over 45 days of the BEDS, Bacteroidetes and Firmicutes were enriched on the biocathode. Putative denitrifying Clostridia belonging to the Firmicutes were distinctively detected on the biocathode via phylogenetic analysis and appeared to be ubiquitous in various autotrophic, heterotrophic, and BEDSs. Elucidation of the bacterial community composition in the denitrification system deepens our knowledge about the bacteria involved in bio-electrochemical denitrification.

AB - Bacterial community composition was investigated in a biocathode of an external-powered bio-electrochemical denitrifying system (BEDS), by analyzing 16S rRNA gene sequences determined from a high-throughput 454 pyrosequencing technology. The bacterial community of the inoculum sludge was mainly composed of Proteobacteria, Bacteroidetes, and Acidobacteria. Over 45 days of the BEDS, Bacteroidetes and Firmicutes were enriched on the biocathode. Putative denitrifying Clostridia belonging to the Firmicutes were distinctively detected on the biocathode via phylogenetic analysis and appeared to be ubiquitous in various autotrophic, heterotrophic, and BEDSs. Elucidation of the bacterial community composition in the denitrification system deepens our knowledge about the bacteria involved in bio-electrochemical denitrification.

KW - Bio-electrochemical denitrification

KW - Clostridia

KW - Denitrification

KW - Firmicutes

KW - Microbial fuel cell

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ER -

Enrichment of Clostridia during the operation of an external-powered bio-electrochemical denitrification system

Abstract

Keywords

ASJC Scopus subject areas

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