Abstract
A packed-bed reactor (length 6.5 cm; id 4.65 mm) has been used to enrich docosahexaenoic acid (DHA) via the lipase-catalyzed esterification of the fatty acid from tuna oil with ethanol. Lipozyme RM IM (from Rhizomucor miehei) was used for the esterification reaction because of its ability to discriminate between different fatty acids, and several reaction parameters, including the temperature, molar ratio of substrates, and water content were explored as a function of residence time. In this way, the optimum conditions for the enrichment process were determined to be a temperature of 20 °C, a molar ratio of 1:5 (i.e., fatty acid to ethanol), and a water content of 1.0 % (based on the total substrate weight). Under these conditions, a residence time of 90 min gave a DHA concentration of 70 wt% and a DHA recovery yield of 87 wt% in the residual fatty acid fraction.
| Original language | English |
|---|---|
| Pages (from-to) | 1877-1884 |
| Number of pages | 8 |
| Journal | JAOCS, Journal of the American Oil Chemists' Society |
| Volume | 91 |
| Issue number | 11 |
| DOIs | |
| Publication status | Published - 2014 Jan 1 |
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ASJC Scopus subject areas
- Chemical Engineering(all)
- Organic Chemistry
Cite this
Enrichment of DHA from tuna oil in a packed bed reactor via lipase-catalyzed esterification. / Hong, Seung In; Ma, Na; No, Da Som; Choi, Nakyung; Baik, Ji Yeon; Kim, Chong Tai; Kim, Yangha; Chang, Eugene; Kim, In-Hwan.
In: JAOCS, Journal of the American Oil Chemists' Society, Vol. 91, No. 11, 01.01.2014, p. 1877-1884.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Enrichment of DHA from tuna oil in a packed bed reactor via lipase-catalyzed esterification
AU - Hong, Seung In
AU - Ma, Na
AU - No, Da Som
AU - Choi, Nakyung
AU - Baik, Ji Yeon
AU - Kim, Chong Tai
AU - Kim, Yangha
AU - Chang, Eugene
AU - Kim, In-Hwan
PY - 2014/1/1
Y1 - 2014/1/1
N2 - A packed-bed reactor (length 6.5 cm; id 4.65 mm) has been used to enrich docosahexaenoic acid (DHA) via the lipase-catalyzed esterification of the fatty acid from tuna oil with ethanol. Lipozyme RM IM (from Rhizomucor miehei) was used for the esterification reaction because of its ability to discriminate between different fatty acids, and several reaction parameters, including the temperature, molar ratio of substrates, and water content were explored as a function of residence time. In this way, the optimum conditions for the enrichment process were determined to be a temperature of 20 °C, a molar ratio of 1:5 (i.e., fatty acid to ethanol), and a water content of 1.0 % (based on the total substrate weight). Under these conditions, a residence time of 90 min gave a DHA concentration of 70 wt% and a DHA recovery yield of 87 wt% in the residual fatty acid fraction.
AB - A packed-bed reactor (length 6.5 cm; id 4.65 mm) has been used to enrich docosahexaenoic acid (DHA) via the lipase-catalyzed esterification of the fatty acid from tuna oil with ethanol. Lipozyme RM IM (from Rhizomucor miehei) was used for the esterification reaction because of its ability to discriminate between different fatty acids, and several reaction parameters, including the temperature, molar ratio of substrates, and water content were explored as a function of residence time. In this way, the optimum conditions for the enrichment process were determined to be a temperature of 20 °C, a molar ratio of 1:5 (i.e., fatty acid to ethanol), and a water content of 1.0 % (based on the total substrate weight). Under these conditions, a residence time of 90 min gave a DHA concentration of 70 wt% and a DHA recovery yield of 87 wt% in the residual fatty acid fraction.
KW - Docosahexaenoic acid (DHA)
KW - Ethanol
KW - Lipozyme RM IM (from Rhizomucor miehei)
KW - Packed-bed reactor (PBR)
KW - Tuna oil fatty acid
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U2 - 10.1007/s11746-014-2536-9
DO - 10.1007/s11746-014-2536-9
M3 - Article
VL - 91
SP - 1877
EP - 1884
JO - Oil & Soap
JF - Oil & Soap
SN - 0003-021X
IS - 11
ER -