TY - JOUR
T1 - Enrichment of docosahexaenoic acid from tuna oil via lipase-mediated esterification under pressurized carbon dioxide
AU - Ma, Na
AU - Hong, Seung In
AU - Zhao, Tingting
AU - No, Da Som
AU - Kim, Chong Tai
AU - Kim, Yangha
AU - Kim, In Hwan
N1 - Funding Information:
This study was supported by the Food High Pressure Technology Development Project, Korea Food Research Institute, Korea .
PY - 2014/3
Y1 - 2014/3
N2 - This study focused on the use of pressurized CO2 as a reaction medium for the enrichment of docosahexaenoic acid (DHA) from tuna oil fatty acids via lipase-mediated esterification. Of the three lipases tested, Lipozyme RM IM from Rhizomucor miehei was selected for further study. Enzyme loading, water addition, and reaction time were also explored. Near-supercritical CO 2, prepared at 25 C and 8.3 MPa, was the most effective reagent tested for enriching DHA from the residual fatty acid fraction. In addition to near-supercritical CO2, optimal conditions included addition of 0.2 wt% (based on total substrates) water, enzyme loading of 5 wt% (based on total substrates), and a reaction time of 18 h. The DHA concentration and recovery yield for the residual fatty acid fraction under these optimal conditions were 75.8 wt% and 81 wt%, respectively.
AB - This study focused on the use of pressurized CO2 as a reaction medium for the enrichment of docosahexaenoic acid (DHA) from tuna oil fatty acids via lipase-mediated esterification. Of the three lipases tested, Lipozyme RM IM from Rhizomucor miehei was selected for further study. Enzyme loading, water addition, and reaction time were also explored. Near-supercritical CO 2, prepared at 25 C and 8.3 MPa, was the most effective reagent tested for enriching DHA from the residual fatty acid fraction. In addition to near-supercritical CO2, optimal conditions included addition of 0.2 wt% (based on total substrates) water, enzyme loading of 5 wt% (based on total substrates), and a reaction time of 18 h. The DHA concentration and recovery yield for the residual fatty acid fraction under these optimal conditions were 75.8 wt% and 81 wt%, respectively.
KW - Docosahexaenoic acid (DHA)
KW - Ethanol
KW - Lipozyme RM IM (Rhizomucor miehei)
KW - Near-supercritical carbon dioxide
KW - Tuna oil fatty acids
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U2 - 10.1016/j.supflu.2013.12.024
DO - 10.1016/j.supflu.2013.12.024
M3 - Article
AN - SCOPUS:84892933332
SN - 0896-8446
VL - 87
SP - 28
EP - 33
JO - Journal of Supercritical Fluids
JF - Journal of Supercritical Fluids
ER -