Enrichment of docosahexaenoic acid from tuna oil via lipase-mediated esterification under pressurized carbon dioxide

Na Ma, Seung In Hong, Tingting Zhao, Da Som No, Chong Tai Kim, Yangha Kim, In Hwan Kim

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

This study focused on the use of pressurized CO2 as a reaction medium for the enrichment of docosahexaenoic acid (DHA) from tuna oil fatty acids via lipase-mediated esterification. Of the three lipases tested, Lipozyme RM IM from Rhizomucor miehei was selected for further study. Enzyme loading, water addition, and reaction time were also explored. Near-supercritical CO 2, prepared at 25 C and 8.3 MPa, was the most effective reagent tested for enriching DHA from the residual fatty acid fraction. In addition to near-supercritical CO2, optimal conditions included addition of 0.2 wt% (based on total substrates) water, enzyme loading of 5 wt% (based on total substrates), and a reaction time of 18 h. The DHA concentration and recovery yield for the residual fatty acid fraction under these optimal conditions were 75.8 wt% and 81 wt%, respectively.

Original languageEnglish
Pages (from-to)28-33
Number of pages6
JournalJournal of Supercritical Fluids
Volume87
DOIs
Publication statusPublished - 2014 Mar

Keywords

  • Docosahexaenoic acid (DHA)
  • Ethanol
  • Lipozyme RM IM (Rhizomucor miehei)
  • Near-supercritical carbon dioxide
  • Tuna oil fatty acids

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Condensed Matter Physics
  • Physical and Theoretical Chemistry

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