Enrichment of docosahexaenoic acid from tuna oil via lipase-mediated esterification under pressurized carbon dioxide

Na Ma, Seung In Hong, Tingting Zhao, Da Som No, Chong Tai Kim, Yangha Kim, In-Hwan Kim

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

This study focused on the use of pressurized CO2 as a reaction medium for the enrichment of docosahexaenoic acid (DHA) from tuna oil fatty acids via lipase-mediated esterification. Of the three lipases tested, Lipozyme RM IM from Rhizomucor miehei was selected for further study. Enzyme loading, water addition, and reaction time were also explored. Near-supercritical CO 2, prepared at 25 C and 8.3 MPa, was the most effective reagent tested for enriching DHA from the residual fatty acid fraction. In addition to near-supercritical CO2, optimal conditions included addition of 0.2 wt% (based on total substrates) water, enzyme loading of 5 wt% (based on total substrates), and a reaction time of 18 h. The DHA concentration and recovery yield for the residual fatty acid fraction under these optimal conditions were 75.8 wt% and 81 wt%, respectively.

Original languageEnglish
Pages (from-to)28-33
Number of pages6
JournalJournal of Supercritical Fluids
Volume87
DOIs
Publication statusPublished - 2014 Mar 1

Fingerprint

Docosahexaenoic Acids
Lipases
Esterification
fatty acids
Lipase
Fatty acids
Carbon Dioxide
carbon dioxide
Carbon dioxide
Oils
Fatty Acids
oils
reaction time
acids
Acids
enzymes
Enzymes
Water
Substrates
Carbon Monoxide

Keywords

  • Docosahexaenoic acid (DHA)
  • Ethanol
  • Lipozyme RM IM (Rhizomucor miehei)
  • Near-supercritical carbon dioxide
  • Tuna oil fatty acids

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Chemical Engineering(all)
  • Condensed Matter Physics

Cite this

Enrichment of docosahexaenoic acid from tuna oil via lipase-mediated esterification under pressurized carbon dioxide. / Ma, Na; Hong, Seung In; Zhao, Tingting; No, Da Som; Kim, Chong Tai; Kim, Yangha; Kim, In-Hwan.

In: Journal of Supercritical Fluids, Vol. 87, 01.03.2014, p. 28-33.

Research output: Contribution to journalArticle

Ma, Na ; Hong, Seung In ; Zhao, Tingting ; No, Da Som ; Kim, Chong Tai ; Kim, Yangha ; Kim, In-Hwan. / Enrichment of docosahexaenoic acid from tuna oil via lipase-mediated esterification under pressurized carbon dioxide. In: Journal of Supercritical Fluids. 2014 ; Vol. 87. pp. 28-33.
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