Enrichment of erucic acid from crambe oil in a recirculated packed bed reactor via lipase-catalyzed ethanolysis

Da Som No, Tingting Zhao, Byung Hee Kim, Hee Don Choi, In Hwan Kim

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10 Citations (Scopus)


Erucic acid enrichment from crambe oil was accomplished in a recirculated packed bed (RPBR) reactor via ethanolysis using Novozym 435 lipase from Candida antarctica as a biocatalyst. The content of erucic acid of the crambe oil used was 59.8 mol% The erucic acid was located predominantly in the sn-1,3 position of the triacylglycerol of the crambe oil. Novozym 435 used in this study has selectivity toward fatty acids in sn-1,3 position of triglyceride in excess ethanol. The effects of reaction temperature, molar ratio, and residence time of the substrate in RPBR on the enrichment of erucic acid as a function of reaction time were studied. The optimal temperature, molar ratio (crambe oil to ethanol), and residence time of the substrate in RPBR were 45 °C, 1:60, and 4 min, respectively.

Original languageEnglish
Pages (from-to)6-10
Number of pages5
JournalJournal of Molecular Catalysis B: Enzymatic
Publication statusPublished - 2013 Mar 1



  • Candida antartica
  • Crambe oil
  • Erucic acid
  • Ethanolysis
  • Recirculated packed bed reactor (RPBR)

ASJC Scopus subject areas

  • Catalysis
  • Bioengineering
  • Biochemistry
  • Process Chemistry and Technology

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