Enrichment of lecithin with n-3 fatty acids by acidolysis using immobilized phospholipase A1

Hugo S. Garcia, In-Hwan Kim, Arnolde Lopez-Hernandez, Charles G. Hill

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

A commercial phospholipase A1 (Lecltase® Ultra) was Immobilized by physical adsorption on Duolite®and then used to mediate the incorporation of omega-3 fatty acids into lecithin. Adsorption isotherms showed that 12 h of contact were sufficient to deposit most of the enzyme onto the carrier. A pH of 7 and 50°C were the best conditions for adsorption. Reaction mixtures consisting of lecithin and a saponified fish oil concentrate (78.4 mol % EPA+DPA+DHA) were prepared at molar ratios ranging from 1:2 to 1:10. Typically 2 g of total substrates and 200 mg of enzyme preparation were employed in batch reactor trials. The fastest reaction rates were observed when a substrate mole ratio of 1:8 (lecithin:total fatty acids) was employed. Use of the enzyme preparation dried at pH 8 and reaction temperatures of 50 and 60°C produced the greatest extent of incorporation of the indicated n-3 fatty acids into the phospholipid after 24h of reaction.

Original languageEnglish
Pages (from-to)368-374
Number of pages7
JournalGrasas y Aceites
Volume59
Issue number4
Publication statusPublished - 2008 Nov 6

Fingerprint

phospholipase A1
Phospholipases A1
acidolysis
Lecithins
Omega-3 Fatty Acids
phosphatidylcholines
omega-3 fatty acids
Adsorption
adsorption
Enzymes
enzymes
Fish Oils
Batch reactors
Substrates
fish oils
Adsorption isotherms
Reaction rates
Phospholipids
phospholipids
Fatty Acids

Keywords

  • Fish oil
  • Interesterification
  • Omega-3
  • Phospholipids

ASJC Scopus subject areas

  • Food Science
  • Organic Chemistry

Cite this

Enrichment of lecithin with n-3 fatty acids by acidolysis using immobilized phospholipase A1. / Garcia, Hugo S.; Kim, In-Hwan; Lopez-Hernandez, Arnolde; Hill, Charles G.

In: Grasas y Aceites, Vol. 59, No. 4, 06.11.2008, p. 368-374.

Research output: Contribution to journalArticle

Garcia, HS, Kim, I-H, Lopez-Hernandez, A & Hill, CG 2008, 'Enrichment of lecithin with n-3 fatty acids by acidolysis using immobilized phospholipase A1', Grasas y Aceites, vol. 59, no. 4, pp. 368-374.
Garcia, Hugo S. ; Kim, In-Hwan ; Lopez-Hernandez, Arnolde ; Hill, Charles G. / Enrichment of lecithin with n-3 fatty acids by acidolysis using immobilized phospholipase A1. In: Grasas y Aceites. 2008 ; Vol. 59, No. 4. pp. 368-374.
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