Abstract
A commercial phospholipase A1 (Lecltase® Ultra) was Immobilized by physical adsorption on Duolite®and then used to mediate the incorporation of omega-3 fatty acids into lecithin. Adsorption isotherms showed that 12 h of contact were sufficient to deposit most of the enzyme onto the carrier. A pH of 7 and 50°C were the best conditions for adsorption. Reaction mixtures consisting of lecithin and a saponified fish oil concentrate (78.4 mol % EPA+DPA+DHA) were prepared at molar ratios ranging from 1:2 to 1:10. Typically 2 g of total substrates and 200 mg of enzyme preparation were employed in batch reactor trials. The fastest reaction rates were observed when a substrate mole ratio of 1:8 (lecithin:total fatty acids) was employed. Use of the enzyme preparation dried at pH 8 and reaction temperatures of 50 and 60°C produced the greatest extent of incorporation of the indicated n-3 fatty acids into the phospholipid after 24h of reaction.
| Original language | English |
|---|---|
| Pages (from-to) | 368-374 |
| Number of pages | 7 |
| Journal | Grasas y Aceites |
| Volume | 59 |
| Issue number | 4 |
| Publication status | Published - 2008 Nov 6 |
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Keywords
- Fish oil
- Interesterification
- Omega-3
- Phospholipids
ASJC Scopus subject areas
- Food Science
- Organic Chemistry
Cite this
Enrichment of lecithin with n-3 fatty acids by acidolysis using immobilized phospholipase A1. / Garcia, Hugo S.; Kim, In-Hwan; Lopez-Hernandez, Arnolde; Hill, Charles G.
In: Grasas y Aceites, Vol. 59, No. 4, 06.11.2008, p. 368-374.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Enrichment of lecithin with n-3 fatty acids by acidolysis using immobilized phospholipase A1
AU - Garcia, Hugo S.
AU - Kim, In-Hwan
AU - Lopez-Hernandez, Arnolde
AU - Hill, Charles G.
PY - 2008/11/6
Y1 - 2008/11/6
N2 - A commercial phospholipase A1 (Lecltase® Ultra) was Immobilized by physical adsorption on Duolite®and then used to mediate the incorporation of omega-3 fatty acids into lecithin. Adsorption isotherms showed that 12 h of contact were sufficient to deposit most of the enzyme onto the carrier. A pH of 7 and 50°C were the best conditions for adsorption. Reaction mixtures consisting of lecithin and a saponified fish oil concentrate (78.4 mol % EPA+DPA+DHA) were prepared at molar ratios ranging from 1:2 to 1:10. Typically 2 g of total substrates and 200 mg of enzyme preparation were employed in batch reactor trials. The fastest reaction rates were observed when a substrate mole ratio of 1:8 (lecithin:total fatty acids) was employed. Use of the enzyme preparation dried at pH 8 and reaction temperatures of 50 and 60°C produced the greatest extent of incorporation of the indicated n-3 fatty acids into the phospholipid after 24h of reaction.
AB - A commercial phospholipase A1 (Lecltase® Ultra) was Immobilized by physical adsorption on Duolite®and then used to mediate the incorporation of omega-3 fatty acids into lecithin. Adsorption isotherms showed that 12 h of contact were sufficient to deposit most of the enzyme onto the carrier. A pH of 7 and 50°C were the best conditions for adsorption. Reaction mixtures consisting of lecithin and a saponified fish oil concentrate (78.4 mol % EPA+DPA+DHA) were prepared at molar ratios ranging from 1:2 to 1:10. Typically 2 g of total substrates and 200 mg of enzyme preparation were employed in batch reactor trials. The fastest reaction rates were observed when a substrate mole ratio of 1:8 (lecithin:total fatty acids) was employed. Use of the enzyme preparation dried at pH 8 and reaction temperatures of 50 and 60°C produced the greatest extent of incorporation of the indicated n-3 fatty acids into the phospholipid after 24h of reaction.
KW - Fish oil
KW - Interesterification
KW - Omega-3
KW - Phospholipids
UR - http://www.scopus.com/inward/record.url?scp=55149107158&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=55149107158&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:55149107158
VL - 59
SP - 368
EP - 374
JO - Grasas y Aceites
JF - Grasas y Aceites
SN - 0017-3495
IS - 4
ER -