Enrichment of lecithin with n-3 fatty acids by acidolysis using immobilized phospholipase A1

Hugo S. Garcia, In Hwan Kim, Arnolde Lopez-Hernandez, Charles G. Hill

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

A commercial phospholipase A1 (Lecltase® Ultra) was Immobilized by physical adsorption on Duolite®and then used to mediate the incorporation of omega-3 fatty acids into lecithin. Adsorption isotherms showed that 12 h of contact were sufficient to deposit most of the enzyme onto the carrier. A pH of 7 and 50°C were the best conditions for adsorption. Reaction mixtures consisting of lecithin and a saponified fish oil concentrate (78.4 mol % EPA+DPA+DHA) were prepared at molar ratios ranging from 1:2 to 1:10. Typically 2 g of total substrates and 200 mg of enzyme preparation were employed in batch reactor trials. The fastest reaction rates were observed when a substrate mole ratio of 1:8 (lecithin:total fatty acids) was employed. Use of the enzyme preparation dried at pH 8 and reaction temperatures of 50 and 60°C produced the greatest extent of incorporation of the indicated n-3 fatty acids into the phospholipid after 24h of reaction.

Original languageEnglish
Pages (from-to)368-374
Number of pages7
JournalGrasas y Aceites
Volume59
Issue number4
DOIs
Publication statusPublished - 2008 Jan 1

Keywords

  • Fish oil
  • Interesterification
  • Omega-3
  • Phospholipids

ASJC Scopus subject areas

  • Food Science
  • Organic Chemistry

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