Enrichment of N-terminal sulfonated peptides by a water-soluble fullerene derivative and its applications to highly efficient proteomics

Yong Ho Lee, Joong Won Shin, Seungwan Ryu, Sang Won Lee, Chang Hoon Lee, Kwangyeol Lee

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Recent studies have shown that N-terminal sulfonation of tryptic peptides by various sulfonating molecules greatly improves their post-source decay processes (e.g., in matrix-assisted laser desorption ionization) or the gas phase fragmentation processes (e.g., in tandem mass spectrometer), enhancing the ability to identify their sequences de novo. In the present work, we have demonstrated that incorporation of water-soluble C60-N,N- dimethylpyrrolidinium iodide selectively precipitates the 4-sulfophenyl isothiocyanate-modified peptide (SPITC-GGYR, SPITC-ASHLGLAR) by forming a noncovalent ion pair to the SO3 - group of the SPITC, and thereby the C60 derivative can be utilized to enrich the modified peptide. Electrospray ionization (ESI) mass analyses show that the cationic SPITC-GGYR and SPITC-ASHLGLAR species are well separated from unmodified peptides and the modified peptides are subsequently detached from the C 60 derivative upon using an acidic solution.

Original languageEnglish
Pages (from-to)140-144
Number of pages5
JournalAnalytica Chimica Acta
Volume556
Issue number1
DOIs
Publication statusPublished - 2006 Jan 18

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Keywords

  • De novo peptide sequencing
  • Liquid chromatography
  • N-terminal sulfonation
  • Tandem mass spectrometry
  • Water-soluble fullerene derivative

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Spectroscopy
  • Environmental Chemistry

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