Enrichment of pinolenic acid from pine nut oil via lipase-catalyzed ethanolysis with an immobilized Candida antarctica lipase

Bo Mi Lee, Jong Hun Choi, Seung In Hong, Sung Won Yoon, Byung Hee Kim, Chong Tai Kim, Chul Jin Kim, Yangha Kim, In Hwan Kim

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Pinolenic acid (PLA) enrichment as an ethyl ester from pine nut oil was successfully accomplished in a batch reactor by lipase-catalyzed ethanolysis using Novozym 435 lipase from Candida antarctica as a biocatalyst. PLA is predominantly an sn-3 substituent of the pine nut oil triacylglycerol (TAG), where it accounts for about 39 mol% of the fatty acids esterified at that position. In the presence of ethanol, Novozym 435 exhibited sn-3 regiospecificity with respect to the TAG of pine nut oil. The effect of the molar ratio of reactants on PLA enrichment by ethanolysis was investigated. The molar ratios of pine nut oil to ethanol were varied from 1:20 to 1:100. A fatty acid ethyl ester (FAEE) fraction with higher PLA content was obtained in the early stage of the reaction, although the yield of PLA was small. However, the PLA content of the FAEEs decreased with increasing reaction time, while the yield of PLA increased. The molar ratio of pine nut oil to ethanol that produced the optimum content and yield of PLA in FAEEs was 1:80.

Original languageEnglish
Pages (from-to)155-160
Number of pages6
JournalBiocatalysis and Biotransformation
Issue number4
Publication statusPublished - 2011 Aug 1



  • Candida antartica
  • Ethanolysis
  • Fatty acid ethyl ester
  • Lipase
  • Pine nut oil
  • Pinolenic acid

ASJC Scopus subject areas

  • Biotechnology
  • Catalysis
  • Biochemistry

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