Abstract
This paper reports a novel method for producing glucose oxidase-nanocomposites by entrapping cross-linked glucose oxidase (GOx) aggregates within a graphitized mesoporous carbon (GMC) network. Entrapment was achieved by utilizing the strong self-aggregation tendency of GMC in aqueous buffer solution to form carbon networks. Using confocal microscopy and TEM, GOx-GMC nanocomposites were visualized. The electrochemical properties of GOx-GMC nanocomposites were studied by means of cyclic voltammograms, chronoamperometric and potentiostatic tests. Results therefrom suggested that the GOx-GMC nanocomposites offer a high electrical conductivity with the maximum electron transfer rate constant estimated at 5.16 ± 0.61 s-1. Furthermore, thermally treating the GOx-GMC nanocomposite and GOx aggregates at 60 °C for four hours, both samples maintained 99% of their initial activity, while the free GOx were completely deactivated. These performances suggested that our nanocomposite structure offered both improved electrochemical performance and stability by combining the high electrical conductivity offered by the GMC network with the high enzyme loading and stability offered by the cross-linked GOx aggregates. The GOx-GMC nanocomposite's electrochemical activity towards glucose oxidation was also investigated by using an enzymatic biofuel cell without artificial mediators, producing a power density of up to 22.4 μW cm-2 at 0.24 V.
| Original language | English |
|---|---|
| Pages (from-to) | 26-34 |
| Number of pages | 9 |
| Journal | Enzyme and Microbial Technology |
| Volume | 90 |
| DOIs | |
| Publication status | Published - 2016 Aug 1 |
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Keywords
- Cross-linked glucose oxidase aggregates
- Efficient electron transfer
- Enzymatic biofuel cells
- Glucose oxidase
- Graphitized mesoporous carbons
ASJC Scopus subject areas
- Biochemistry
- Biotechnology
- Applied Microbiology and Biotechnology
Cite this
Entrapping cross-linked glucose oxidase aggregates within a graphitized mesoporous carbon network for enzymatic biofuel cells. / Garcia-Perez, Tsai; Hong, Sung Gil; Kim, Jungbae; Ha, Su.
In: Enzyme and Microbial Technology, Vol. 90, 01.08.2016, p. 26-34.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Entrapping cross-linked glucose oxidase aggregates within a graphitized mesoporous carbon network for enzymatic biofuel cells
AU - Garcia-Perez, Tsai
AU - Hong, Sung Gil
AU - Kim, Jungbae
AU - Ha, Su
PY - 2016/8/1
Y1 - 2016/8/1
N2 - This paper reports a novel method for producing glucose oxidase-nanocomposites by entrapping cross-linked glucose oxidase (GOx) aggregates within a graphitized mesoporous carbon (GMC) network. Entrapment was achieved by utilizing the strong self-aggregation tendency of GMC in aqueous buffer solution to form carbon networks. Using confocal microscopy and TEM, GOx-GMC nanocomposites were visualized. The electrochemical properties of GOx-GMC nanocomposites were studied by means of cyclic voltammograms, chronoamperometric and potentiostatic tests. Results therefrom suggested that the GOx-GMC nanocomposites offer a high electrical conductivity with the maximum electron transfer rate constant estimated at 5.16 ± 0.61 s-1. Furthermore, thermally treating the GOx-GMC nanocomposite and GOx aggregates at 60 °C for four hours, both samples maintained 99% of their initial activity, while the free GOx were completely deactivated. These performances suggested that our nanocomposite structure offered both improved electrochemical performance and stability by combining the high electrical conductivity offered by the GMC network with the high enzyme loading and stability offered by the cross-linked GOx aggregates. The GOx-GMC nanocomposite's electrochemical activity towards glucose oxidation was also investigated by using an enzymatic biofuel cell without artificial mediators, producing a power density of up to 22.4 μW cm-2 at 0.24 V.
AB - This paper reports a novel method for producing glucose oxidase-nanocomposites by entrapping cross-linked glucose oxidase (GOx) aggregates within a graphitized mesoporous carbon (GMC) network. Entrapment was achieved by utilizing the strong self-aggregation tendency of GMC in aqueous buffer solution to form carbon networks. Using confocal microscopy and TEM, GOx-GMC nanocomposites were visualized. The electrochemical properties of GOx-GMC nanocomposites were studied by means of cyclic voltammograms, chronoamperometric and potentiostatic tests. Results therefrom suggested that the GOx-GMC nanocomposites offer a high electrical conductivity with the maximum electron transfer rate constant estimated at 5.16 ± 0.61 s-1. Furthermore, thermally treating the GOx-GMC nanocomposite and GOx aggregates at 60 °C for four hours, both samples maintained 99% of their initial activity, while the free GOx were completely deactivated. These performances suggested that our nanocomposite structure offered both improved electrochemical performance and stability by combining the high electrical conductivity offered by the GMC network with the high enzyme loading and stability offered by the cross-linked GOx aggregates. The GOx-GMC nanocomposite's electrochemical activity towards glucose oxidation was also investigated by using an enzymatic biofuel cell without artificial mediators, producing a power density of up to 22.4 μW cm-2 at 0.24 V.
KW - Cross-linked glucose oxidase aggregates
KW - Efficient electron transfer
KW - Enzymatic biofuel cells
KW - Glucose oxidase
KW - Graphitized mesoporous carbons
UR - http://www.scopus.com/inward/record.url?scp=84964895369&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84964895369&partnerID=8YFLogxK
U2 - 10.1016/j.enzmictec.2016.04.010
DO - 10.1016/j.enzmictec.2016.04.010
M3 - Article
C2 - 27241289
AN - SCOPUS:84964895369
VL - 90
SP - 26
EP - 34
JO - Enzyme and Microbial Technology
JF - Enzyme and Microbial Technology
SN - 0141-0229
ER -