Environment-friendly mild heat and relative humidity treatment protects sprout seeds (radish, mung bean, mustard, and alfalfa) against various foodborne pathogens

Se Han Kim, Min-Suk Rhee

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The utility of a novel technology based on heat and relative humidity (RH) was examined; the method was used to treat experimentally contaminated radish, mung bean, mustard, and alfalfa seeds. Seeds were inoculated with high (ca. 7 log CFU/g) or low (ca. 3 log CFU/g) levels of Escherichia coli O157:H7, Salmonella Typhimurium, and Listeria monocytogenes, and treated at 65 °C/40% RH for 8, 15, or 22 h. The 15 h treatment reduced the numbers of E. coli O157:H7 and S. Typhimurium on all seeds to below the detection limit (10 CFU/g), whereas longer treatment (up to 22 h) was needed for L. monocytogenes. At 8 h, L. monocytogenes on mung bean was significantly less susceptible to combined treatment that L. monocytogenes on other seeds (P < 0.05). Overall, the 22 h treatment eliminated E. coli O157:H7 from radish and mustard seeds, and L. monocytogenes from mustard and alfalfa seeds; the treatment had no significant effect on the viability of radish, mung bean, or alfalfa seeds (P > 0.05). However, the mustard seed viability fell by about 9.8%. These findings indicate that the environmentally friendly technology is a wide spectrum method of decontaminating sprout seeds, with little concomitant reduction in seed quality.

Original languageEnglish
Pages (from-to)17-22
Number of pages6
JournalFood Control
Volume93
DOIs
Publication statusPublished - 2018 Nov 1

Fingerprint

Raphanus
sprouts (food)
Mustard Plant
Medicago sativa
food pathogens
radishes
mustard (condiment)
Humidity
mung beans
alfalfa
Seeds
relative humidity
Hot Temperature
Listeria monocytogenes
heat
seeds
Escherichia coli O157
Salmonella Typhimurium
Salmonella typhimurium
mustard seed

Keywords

  • Escherichia coli O157:H7
  • Heat and relative humidity
  • Listeria monocytogenes
  • Salmonella Typhimurium
  • Sprout seeds

ASJC Scopus subject areas

  • Biotechnology
  • Food Science

Cite this

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title = "Environment-friendly mild heat and relative humidity treatment protects sprout seeds (radish, mung bean, mustard, and alfalfa) against various foodborne pathogens",
abstract = "The utility of a novel technology based on heat and relative humidity (RH) was examined; the method was used to treat experimentally contaminated radish, mung bean, mustard, and alfalfa seeds. Seeds were inoculated with high (ca. 7 log CFU/g) or low (ca. 3 log CFU/g) levels of Escherichia coli O157:H7, Salmonella Typhimurium, and Listeria monocytogenes, and treated at 65 °C/40{\%} RH for 8, 15, or 22 h. The 15 h treatment reduced the numbers of E. coli O157:H7 and S. Typhimurium on all seeds to below the detection limit (10 CFU/g), whereas longer treatment (up to 22 h) was needed for L. monocytogenes. At 8 h, L. monocytogenes on mung bean was significantly less susceptible to combined treatment that L. monocytogenes on other seeds (P < 0.05). Overall, the 22 h treatment eliminated E. coli O157:H7 from radish and mustard seeds, and L. monocytogenes from mustard and alfalfa seeds; the treatment had no significant effect on the viability of radish, mung bean, or alfalfa seeds (P > 0.05). However, the mustard seed viability fell by about 9.8{\%}. These findings indicate that the environmentally friendly technology is a wide spectrum method of decontaminating sprout seeds, with little concomitant reduction in seed quality.",
keywords = "Escherichia coli O157:H7, Heat and relative humidity, Listeria monocytogenes, Salmonella Typhimurium, Sprout seeds",
author = "Kim, {Se Han} and Min-Suk Rhee",
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N2 - The utility of a novel technology based on heat and relative humidity (RH) was examined; the method was used to treat experimentally contaminated radish, mung bean, mustard, and alfalfa seeds. Seeds were inoculated with high (ca. 7 log CFU/g) or low (ca. 3 log CFU/g) levels of Escherichia coli O157:H7, Salmonella Typhimurium, and Listeria monocytogenes, and treated at 65 °C/40% RH for 8, 15, or 22 h. The 15 h treatment reduced the numbers of E. coli O157:H7 and S. Typhimurium on all seeds to below the detection limit (10 CFU/g), whereas longer treatment (up to 22 h) was needed for L. monocytogenes. At 8 h, L. monocytogenes on mung bean was significantly less susceptible to combined treatment that L. monocytogenes on other seeds (P < 0.05). Overall, the 22 h treatment eliminated E. coli O157:H7 from radish and mustard seeds, and L. monocytogenes from mustard and alfalfa seeds; the treatment had no significant effect on the viability of radish, mung bean, or alfalfa seeds (P > 0.05). However, the mustard seed viability fell by about 9.8%. These findings indicate that the environmentally friendly technology is a wide spectrum method of decontaminating sprout seeds, with little concomitant reduction in seed quality.

AB - The utility of a novel technology based on heat and relative humidity (RH) was examined; the method was used to treat experimentally contaminated radish, mung bean, mustard, and alfalfa seeds. Seeds were inoculated with high (ca. 7 log CFU/g) or low (ca. 3 log CFU/g) levels of Escherichia coli O157:H7, Salmonella Typhimurium, and Listeria monocytogenes, and treated at 65 °C/40% RH for 8, 15, or 22 h. The 15 h treatment reduced the numbers of E. coli O157:H7 and S. Typhimurium on all seeds to below the detection limit (10 CFU/g), whereas longer treatment (up to 22 h) was needed for L. monocytogenes. At 8 h, L. monocytogenes on mung bean was significantly less susceptible to combined treatment that L. monocytogenes on other seeds (P < 0.05). Overall, the 22 h treatment eliminated E. coli O157:H7 from radish and mustard seeds, and L. monocytogenes from mustard and alfalfa seeds; the treatment had no significant effect on the viability of radish, mung bean, or alfalfa seeds (P > 0.05). However, the mustard seed viability fell by about 9.8%. These findings indicate that the environmentally friendly technology is a wide spectrum method of decontaminating sprout seeds, with little concomitant reduction in seed quality.

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