Abstract
Hydroxy fatty acids (HFAs), originally found in small amount mainly from plant systems, are well known to have special properties such as higher viscosity and reactivity compared with other normal fatty acids. Recently, various microbial strains were tested to produce HFAs from different unsaturated fatty acids. Among those microbial strains tested, Pseudomonas aeruginosa PR3 are well known to utilize various unsaturated fatty acids to produce mono-, di-, and tri-HFAs. Previously, we reported that strain PR3 could utilize triolein as a substrate for the production of 7,10-dihydroxy-8(E)-octadecenoic acid (DOD) via the induction of lipase activity (Chang et al., Appl Microbiol Biotechnol, 74:301-306, 2007). In this study, we focused on the development of the optimal environmental conditions for DOD production from triolein by PR3. Optimal initial medium pH and incubation temperature were pH 8.0 and 25°C, respectively. Magnesium ion was essentially required for DOD production. Optimal inoculum size, time for substrate addition, and substrate concentration were 1%, 12 to 24 h, and 300 mg, respectively.
| Original language | English |
|---|---|
| Pages (from-to) | 581-586 |
| Number of pages | 6 |
| Journal | Applied Microbiology and Biotechnology |
| Volume | 78 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2008 Mar 1 |
Fingerprint
Keywords
- Bioconversion
- Hydroxy fatty acid
- Optimization
- Pseudomonas aeruginosa
- Triolein
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Microbiology
- Microbiology (medical)
Cite this
Environmental optimization for bioconversion of triolein into 7,10-dihydroxy-8(E)-octadecenoic acid by Pseudomonas aeruginosa PR3. / Chang, In A.; Bae, Jae H.; Suh, Min Jung; Kim, In-Hwan; Hou, Ching T.; Kim, Hak Ryul.
In: Applied Microbiology and Biotechnology, Vol. 78, No. 4, 01.03.2008, p. 581-586.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Environmental optimization for bioconversion of triolein into 7,10-dihydroxy-8(E)-octadecenoic acid by Pseudomonas aeruginosa PR3
AU - Chang, In A.
AU - Bae, Jae H.
AU - Suh, Min Jung
AU - Kim, In-Hwan
AU - Hou, Ching T.
AU - Kim, Hak Ryul
PY - 2008/3/1
Y1 - 2008/3/1
N2 - Hydroxy fatty acids (HFAs), originally found in small amount mainly from plant systems, are well known to have special properties such as higher viscosity and reactivity compared with other normal fatty acids. Recently, various microbial strains were tested to produce HFAs from different unsaturated fatty acids. Among those microbial strains tested, Pseudomonas aeruginosa PR3 are well known to utilize various unsaturated fatty acids to produce mono-, di-, and tri-HFAs. Previously, we reported that strain PR3 could utilize triolein as a substrate for the production of 7,10-dihydroxy-8(E)-octadecenoic acid (DOD) via the induction of lipase activity (Chang et al., Appl Microbiol Biotechnol, 74:301-306, 2007). In this study, we focused on the development of the optimal environmental conditions for DOD production from triolein by PR3. Optimal initial medium pH and incubation temperature were pH 8.0 and 25°C, respectively. Magnesium ion was essentially required for DOD production. Optimal inoculum size, time for substrate addition, and substrate concentration were 1%, 12 to 24 h, and 300 mg, respectively.
AB - Hydroxy fatty acids (HFAs), originally found in small amount mainly from plant systems, are well known to have special properties such as higher viscosity and reactivity compared with other normal fatty acids. Recently, various microbial strains were tested to produce HFAs from different unsaturated fatty acids. Among those microbial strains tested, Pseudomonas aeruginosa PR3 are well known to utilize various unsaturated fatty acids to produce mono-, di-, and tri-HFAs. Previously, we reported that strain PR3 could utilize triolein as a substrate for the production of 7,10-dihydroxy-8(E)-octadecenoic acid (DOD) via the induction of lipase activity (Chang et al., Appl Microbiol Biotechnol, 74:301-306, 2007). In this study, we focused on the development of the optimal environmental conditions for DOD production from triolein by PR3. Optimal initial medium pH and incubation temperature were pH 8.0 and 25°C, respectively. Magnesium ion was essentially required for DOD production. Optimal inoculum size, time for substrate addition, and substrate concentration were 1%, 12 to 24 h, and 300 mg, respectively.
KW - Bioconversion
KW - Hydroxy fatty acid
KW - Optimization
KW - Pseudomonas aeruginosa
KW - Triolein
UR - http://www.scopus.com/inward/record.url?scp=40549101861&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=40549101861&partnerID=8YFLogxK
U2 - 10.1007/s00253-007-1342-9
DO - 10.1007/s00253-007-1342-9
M3 - Article
VL - 78
SP - 581
EP - 586
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
SN - 0175-7598
IS - 4
ER -