Enzymatic characteristics of biosynthesis and degradation of poly-β-hydroxybutyrate of alcaligenes latus

Tae Woo Kim, Jin Seo Park, Yong Hyun Lee

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

The enzymatic characteristics of Alcaligenes latus were investigated by measuring the variations of various enzyme activities related to biosynthesis and degradation of poly-β-hydroxybutyrate (PHB) during cultivation. All PHB biosynthetic enzymes, β-ketothiolase, acetoacetyl-CoA reductase, and PHB synthase, were activated gradually at the PHB accumulation stage, and the PHB synthase showed the highest value among three enzymes. This indicates that the rate of PHB biosynthesis is mainly controlled by either β-ketothiolase or acetoacetyl-CoA reductase rather than PHB synthase. The enzymatic activities related to the degradation of PHB were also measured, and the degradation of PHB was controlled by the activity of PHB depolymerase. The effect of supplements of metabolic regulators, citrate and tyrosine, was also investigated, and the activity of glucose-6-phosphate dehydrogenase was increased by metabolic regulators, especially by tyrosine. The activities of β-ketothiolase and acetoacetyl-CoA reductase were also activated by citrate and tyrosine, while the activity of PHB depolymerase was depressed. The increased rate and yield of PHB biosynthesis by metabolic regulators may be due to the increment of acetyl-CoA concentration either by the repression of the TCA cycle by citrate through product inhibition or by the activation of sucrose metabolism by the supplemented tyrosine.

Original languageEnglish
Pages (from-to)425-431
Number of pages7
JournalJournal of microbiology and biotechnology
Volume6
Issue number6
Publication statusPublished - 1996 Dec
Externally publishedYes

Keywords

  • Alcaligenes latus
  • Enzymatic characteristics
  • PHB biosynthesis
  • PHB degradation
  • Poly-β-hydroxybutyrate
  • Sugar catabolism
  • Supplements of citrate and tyrosine

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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