Enzymatic glucose biosensor based on porous ZnO/Au electrodes

Xueqiu You, Jungil Park, Yunseok Jang, Soo-Won Kim, James Jungho Pak, Nam Ki Min

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

This paper describes a glucose biosensor based on glucose oxidase (GOx) immobilized on porous ZnO/Au electrodes. The ZnO porous electrodes were fabricated by electrochemical deposition of zinc oxide on patterned Au electrodes using polystyrene (PS) spheres as templates. Uniform pore size and highly ordered ZnO pore arrangement were observed from SEM images. X-ray diffraction (XRD) patterns revealed a single crystalline nature of the porous ZnO. This porous structure provides high enzyme loading capacity and long-term stability. In a pH 7.4 phosphate buffer solution, the positively charged high isoelectric point (IEP) ZnO pores enhance the adsorption of negatively charged low IEP GOx through electrostatic attractive force. Once GOx molecules are immobilized within the ZnO pores, the bottleneck structure resulting from the connected pores hinders leaching of GOx from the pores. The resulting enzymatic biosensor showed a linear detection range from 1mM to 18mM, and sensitivity of 10.89μA/ (mM·cm2) with good selectivity and long-term stability.

Original languageEnglish
Title of host publication2010 IEEE International Conference on Nano/Molecular Medicine and Engineering, IEEE NANOMED 2010
Pages56-59
Number of pages4
DOIs
Publication statusPublished - 2010 Dec 1
Event4th IEEE International Conference on Nano/Molecular Medicine and Engineering, IEEE NANOMED 2010 - Hong Kong/Macau, China
Duration: 2010 Dec 52010 Dec 9

Other

Other4th IEEE International Conference on Nano/Molecular Medicine and Engineering, IEEE NANOMED 2010
CountryChina
CityHong Kong/Macau
Period10/12/510/12/9

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Keywords

  • Enzyme immobilization
  • Glucose biosensor
  • Glucose oxidase
  • ZnO porous structure

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology

Cite this

You, X., Park, J., Jang, Y., Kim, S-W., Pak, J. J., & Min, N. K. (2010). Enzymatic glucose biosensor based on porous ZnO/Au electrodes. In 2010 IEEE International Conference on Nano/Molecular Medicine and Engineering, IEEE NANOMED 2010 (pp. 56-59). [5749805] https://doi.org/10.1109/NANOMED.2010.5749805