Enzymatic hydrolysis of aspen biomass into fermentable sugars by using lignocellulases from Armillaria gemina

Sujit Sadashiv Jagtap, Saurabh Sudha Dhiman, Tae Su Kim, Jinglin Li, Jung Kul Lee, Yun Chan Kang

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

A white rot fungus, identified as Armillaria gemina SKU2114 on the basis of morphological and phylogenetic analyses, was found to secrete efficient lignocellulose-degrading enzymes. The strain showed maximum endoglucanase, cellobiohydrolase, and β-glucosidase activities of 146, 34, and 15. U/mL, respectively, and also secreted xylanase, laccase, mannanase, and lignin peroxidase with activities of 1270, 0.16, 57, and 0.31. U/mL, respectively, when grown with rice straw as a carbon source. Among various plant biomasses tested for saccharification, aspen biomass produced the maximum amount of reducing sugar. Response surface methodology was used to optimize the hydrolysis of aspen biomass to achieve the highest level of sugar production. A maximum saccharification yield of 62% (429. mg/g-substrate) was obtained using Populus tomentiglandulosa biomass after 48. h of hydrolysis. A. gemina was shown to be a good option for use in the production of reducing sugars from lignocellulosic biomass.

Original languageEnglish
Pages (from-to)307-314
Number of pages8
JournalBioresource Technology
Volume133
DOIs
Publication statusPublished - 2013 Jan 1
Externally publishedYes

Fingerprint

Enzymatic hydrolysis
Sugars
hydrolysis
sugar
Biomass
biomass
Saccharification
Hydrolysis
Cellulose 1,4-beta-Cellobiosidase
Glucosidases
Laccase
Cellulase
Straw
Lignin
Fungi
straw
lignin
Carbon
rice
Enzymes

Keywords

  • Armillaria gemina
  • Aspen biomass
  • Lignocellulase
  • Response surface methodology
  • Saccharification]

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

Cite this

Enzymatic hydrolysis of aspen biomass into fermentable sugars by using lignocellulases from Armillaria gemina. / Jagtap, Sujit Sadashiv; Dhiman, Saurabh Sudha; Kim, Tae Su; Li, Jinglin; Lee, Jung Kul; Kang, Yun Chan.

In: Bioresource Technology, Vol. 133, 01.01.2013, p. 307-314.

Research output: Contribution to journalArticle

Jagtap, Sujit Sadashiv ; Dhiman, Saurabh Sudha ; Kim, Tae Su ; Li, Jinglin ; Lee, Jung Kul ; Kang, Yun Chan. / Enzymatic hydrolysis of aspen biomass into fermentable sugars by using lignocellulases from Armillaria gemina. In: Bioresource Technology. 2013 ; Vol. 133. pp. 307-314.
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