TY - JOUR
T1 - Enzymatic hydrolysis of aspen biomass into fermentable sugars by using lignocellulases from Armillaria gemina
AU - Jagtap, Sujit Sadashiv
AU - Dhiman, Saurabh Sudha
AU - Kim, Tae Su
AU - Li, Jinglin
AU - Lee, Jung Kul
AU - Kang, Yun Chan
N1 - Funding Information:
This research was supported by the Converging Research Center Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology ( 2011-50210 ). This work was also supported by the NRF grant funded by the Korea government (2012M3C5A1053339).
PY - 2013/4
Y1 - 2013/4
N2 - A white rot fungus, identified as Armillaria gemina SKU2114 on the basis of morphological and phylogenetic analyses, was found to secrete efficient lignocellulose-degrading enzymes. The strain showed maximum endoglucanase, cellobiohydrolase, and β-glucosidase activities of 146, 34, and 15. U/mL, respectively, and also secreted xylanase, laccase, mannanase, and lignin peroxidase with activities of 1270, 0.16, 57, and 0.31. U/mL, respectively, when grown with rice straw as a carbon source. Among various plant biomasses tested for saccharification, aspen biomass produced the maximum amount of reducing sugar. Response surface methodology was used to optimize the hydrolysis of aspen biomass to achieve the highest level of sugar production. A maximum saccharification yield of 62% (429. mg/g-substrate) was obtained using Populus tomentiglandulosa biomass after 48. h of hydrolysis. A. gemina was shown to be a good option for use in the production of reducing sugars from lignocellulosic biomass.
AB - A white rot fungus, identified as Armillaria gemina SKU2114 on the basis of morphological and phylogenetic analyses, was found to secrete efficient lignocellulose-degrading enzymes. The strain showed maximum endoglucanase, cellobiohydrolase, and β-glucosidase activities of 146, 34, and 15. U/mL, respectively, and also secreted xylanase, laccase, mannanase, and lignin peroxidase with activities of 1270, 0.16, 57, and 0.31. U/mL, respectively, when grown with rice straw as a carbon source. Among various plant biomasses tested for saccharification, aspen biomass produced the maximum amount of reducing sugar. Response surface methodology was used to optimize the hydrolysis of aspen biomass to achieve the highest level of sugar production. A maximum saccharification yield of 62% (429. mg/g-substrate) was obtained using Populus tomentiglandulosa biomass after 48. h of hydrolysis. A. gemina was shown to be a good option for use in the production of reducing sugars from lignocellulosic biomass.
KW - Armillaria gemina
KW - Aspen biomass
KW - Lignocellulase
KW - Response surface methodology
KW - Saccharification]
UR - http://www.scopus.com/inward/record.url?scp=84874361155&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2013.01.118
DO - 10.1016/j.biortech.2013.01.118
M3 - Article
C2 - 23434807
AN - SCOPUS:84874361155
SN - 0960-8524
VL - 133
SP - 307
EP - 314
JO - Bioresource Technology
JF - Bioresource Technology
ER -
