Enzymatic liquefaction of agarose above the sol–gel transition temperature using a thermostable endo-type β-agarase, Aga16B

Jung Hyun Kim, Eun Ju Yun, Nari Seo, Sora Yu, Dong Hyun Kim, Kyung Mun Cho, Hyun Joo An, Jae Han Kim, In-Geol Choi, Kyoung Heon Kim

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The main carbohydrate of red macroalgae is agarose, a heterogeneous polysaccharide composed of d-galactose and 3,6-anhydro-l-galactose. When saccharifying agarose by enzymes, the unique physical properties of agarose, namely the sol–gel transition and the near-insolubility of agarose in water, limit the accessibility of agarose to the enzymes. Due to the lower accessibility of agarose to enzymes in the gel state than to the sol state, it is important to prevent the sol–gel transition by performing the enzymatic liquefaction of agarose at a temperature higher than the sol–gel transition temperature of agarose. In this study, a thermostable endo-type β-agarase, Aga16B, originating from Saccharophagus degradans 2-40T, was characterized and introduced in the liquefaction process. Aga16B was thermostable up to 50 °C and depolymerized agarose mainly into neoagarooligosaccharides with degrees of polymerization 4 and 6. Aga16B was applied to enzymatic liquefaction of agarose at 45 °C, which was above the sol–gel transition temperature of 1 % (w/v) agarose (∼35 °C) when cooling agarose. This is the first systematic demonstration of enzymatic liquefaction of agarose, enabled by determining the sol–gel temperature of agarose under specific conditions and by characterizing the thermostability of an endo-type β-agarase.

Original languageEnglish
Pages (from-to)1-10
Number of pages10
JournalApplied Microbiology and Biotechnology
DOIs
Publication statusAccepted/In press - 2016 Sep 24

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Transition Temperature
Sepharose
agarase
Galactose
Enzymes
Seaweed
Temperature
Polymethyl Methacrylate
Polymerization
Polysaccharides

Keywords

  • Agarase
  • Agarose
  • Enzymatic liquefaction
  • Red macroalgae
  • Saccharification

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Enzymatic liquefaction of agarose above the sol–gel transition temperature using a thermostable endo-type β-agarase, Aga16B. / Kim, Jung Hyun; Yun, Eun Ju; Seo, Nari; Yu, Sora; Kim, Dong Hyun; Cho, Kyung Mun; An, Hyun Joo; Kim, Jae Han; Choi, In-Geol; Kim, Kyoung Heon.

In: Applied Microbiology and Biotechnology, 24.09.2016, p. 1-10.

Research output: Contribution to journalArticle

Kim, Jung Hyun ; Yun, Eun Ju ; Seo, Nari ; Yu, Sora ; Kim, Dong Hyun ; Cho, Kyung Mun ; An, Hyun Joo ; Kim, Jae Han ; Choi, In-Geol ; Kim, Kyoung Heon. / Enzymatic liquefaction of agarose above the sol–gel transition temperature using a thermostable endo-type β-agarase, Aga16B. In: Applied Microbiology and Biotechnology. 2016 ; pp. 1-10.
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