Abstract
The production of ethanol from feedstock other than agriculture materials has been promoted in recent years. Some microalgae can accumulate a high starch content (about 44% of dry base) via photosynthesis. Algal biomass, Chlamydomonas reinhardtii UTEX 90, was converted into a suitable fermentable feedstock by two commercial hydrolytic enzymes. The results showed that almost all starch was released and converted into glucose without steps for the cell wall disruption. Various conditions in the liquefaction and saccharification processes, such as enzyme concentration, pH, temperature, and residence time, have been investigated to obtain an optimum combination using the orthogonal analysis. As a result, approximately 235 mg of ethanol was produced from 1.0 g of algal biomass by a separate hydrolysis and fermentation (SHF) method. The main advantages of this process include the low cost of chemicals, short residence time, and simple equipment system, all of which promote its large-scale application.
| Original language | English |
|---|---|
| Pages (from-to) | 5330-5336 |
| Number of pages | 7 |
| Journal | Bioresource Technology |
| Volume | 101 |
| Issue number | 14 |
| DOIs | |
| Publication status | Published - 2010 Jul 1 |
| Externally published | Yes |
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Keywords
- Chlamydomonas reinhardtii
- Enzymatic pretreatment
- Ethanol
- Microalgae
ASJC Scopus subject areas
- Bioengineering
- Environmental Engineering
- Waste Management and Disposal
Cite this
Enzymatic pretreatment of Chlamydomonas reinhardtii biomass for ethanol production. / Choi, Seung Phill; Nguyen, Minh Thu; Sim, Sang Jun.
In: Bioresource Technology, Vol. 101, No. 14, 01.07.2010, p. 5330-5336.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Enzymatic pretreatment of Chlamydomonas reinhardtii biomass for ethanol production
AU - Choi, Seung Phill
AU - Nguyen, Minh Thu
AU - Sim, Sang Jun
PY - 2010/7/1
Y1 - 2010/7/1
N2 - The production of ethanol from feedstock other than agriculture materials has been promoted in recent years. Some microalgae can accumulate a high starch content (about 44% of dry base) via photosynthesis. Algal biomass, Chlamydomonas reinhardtii UTEX 90, was converted into a suitable fermentable feedstock by two commercial hydrolytic enzymes. The results showed that almost all starch was released and converted into glucose without steps for the cell wall disruption. Various conditions in the liquefaction and saccharification processes, such as enzyme concentration, pH, temperature, and residence time, have been investigated to obtain an optimum combination using the orthogonal analysis. As a result, approximately 235 mg of ethanol was produced from 1.0 g of algal biomass by a separate hydrolysis and fermentation (SHF) method. The main advantages of this process include the low cost of chemicals, short residence time, and simple equipment system, all of which promote its large-scale application.
AB - The production of ethanol from feedstock other than agriculture materials has been promoted in recent years. Some microalgae can accumulate a high starch content (about 44% of dry base) via photosynthesis. Algal biomass, Chlamydomonas reinhardtii UTEX 90, was converted into a suitable fermentable feedstock by two commercial hydrolytic enzymes. The results showed that almost all starch was released and converted into glucose without steps for the cell wall disruption. Various conditions in the liquefaction and saccharification processes, such as enzyme concentration, pH, temperature, and residence time, have been investigated to obtain an optimum combination using the orthogonal analysis. As a result, approximately 235 mg of ethanol was produced from 1.0 g of algal biomass by a separate hydrolysis and fermentation (SHF) method. The main advantages of this process include the low cost of chemicals, short residence time, and simple equipment system, all of which promote its large-scale application.
KW - Chlamydomonas reinhardtii
KW - Enzymatic pretreatment
KW - Ethanol
KW - Microalgae
UR - http://www.scopus.com/inward/record.url?scp=77950341874&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77950341874&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2010.02.026
DO - 10.1016/j.biortech.2010.02.026
M3 - Article
VL - 101
SP - 5330
EP - 5336
JO - Bioresource Technology
JF - Bioresource Technology
SN - 0960-8524
IS - 14
ER -