Enzymatic pretreatment of Chlamydomonas reinhardtii biomass for ethanol production

Seung Phill Choi, Minh Thu Nguyen, Sang Jun Sim

Research output: Contribution to journalArticle

182 Citations (Scopus)

Abstract

The production of ethanol from feedstock other than agriculture materials has been promoted in recent years. Some microalgae can accumulate a high starch content (about 44% of dry base) via photosynthesis. Algal biomass, Chlamydomonas reinhardtii UTEX 90, was converted into a suitable fermentable feedstock by two commercial hydrolytic enzymes. The results showed that almost all starch was released and converted into glucose without steps for the cell wall disruption. Various conditions in the liquefaction and saccharification processes, such as enzyme concentration, pH, temperature, and residence time, have been investigated to obtain an optimum combination using the orthogonal analysis. As a result, approximately 235 mg of ethanol was produced from 1.0 g of algal biomass by a separate hydrolysis and fermentation (SHF) method. The main advantages of this process include the low cost of chemicals, short residence time, and simple equipment system, all of which promote its large-scale application.

Original languageEnglish
Pages (from-to)5330-5336
Number of pages7
JournalBioresource Technology
Volume101
Issue number14
DOIs
Publication statusPublished - 2010 Jul 1
Externally publishedYes

Fingerprint

Starch
starch
Feedstocks
ethanol
residence time
Biomass
Ethanol
Enzymes
enzyme
Saccharification
Photosynthesis
biomass
Liquefaction
liquefaction
Fermentation
Agriculture
fermentation
Glucose
hydrolysis
Hydrolysis

Keywords

  • Chlamydomonas reinhardtii
  • Enzymatic pretreatment
  • Ethanol
  • Microalgae

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Waste Management and Disposal

Cite this

Enzymatic pretreatment of Chlamydomonas reinhardtii biomass for ethanol production. / Choi, Seung Phill; Nguyen, Minh Thu; Sim, Sang Jun.

In: Bioresource Technology, Vol. 101, No. 14, 01.07.2010, p. 5330-5336.

Research output: Contribution to journalArticle

Choi, Seung Phill ; Nguyen, Minh Thu ; Sim, Sang Jun. / Enzymatic pretreatment of Chlamydomonas reinhardtii biomass for ethanol production. In: Bioresource Technology. 2010 ; Vol. 101, No. 14. pp. 5330-5336.
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