Enzymatic synthesis of structured lipids using a novel cold-active lipase from Pichia lynferdii NRRL Y-7723

Hak Ryul Kim, Ching T. Hou, Ki Teak Lee, Byung Hee Kim, In-Hwan Kim

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Structured lipids (SL) were synthesized by the acidolysis of borage oil with caprylic acid using lipases. Six commercial lipases from different sources and a novel lipase from Pichia lynferdii NRRL Y-7723 were screened for their acidolysis activities and Lipozyme RM IM and NRRL Y-7723 lipase were selected to synthesize symmetrical SL since recently NRRL Y-7723 lipase was identified as a novel cold-active lipase. Both lipases showed 1,3-regiospecifity toward the glycerol backbone of borage oil. The effects of enzyme loading and temperature on caprylic acid incorporation into the borage oil were investigated. For Lipozyme RM IM and NRRL Y-7723 lipase, the incorporation of caprylic acid increased as enzyme loading increased up to 4% of total weight of the substrate, but significant increases were not observed when enzyme loading was further increased. The activity of NRRL Y-7723 lipase was higher than that of Lipozyme RM IM in the temperature range between 10 and 20 °C.

Original languageEnglish
Pages (from-to)846-849
Number of pages4
JournalFood Chemistry
Volume122
Issue number3
DOIs
Publication statusPublished - 2010 Oct 1

Fingerprint

borage oil
structured lipids
Pichia
octanoic acid
Lipase
acidolysis
Lipids
synthesis
enzymes
glycerol
temperature
Enzymes
Temperature
Glycerol

Keywords

  • Acidolysis
  • Borage oil
  • Caprylic acid
  • Enzyme loading
  • Novel lipase
  • Regiospecifity
  • Structured lipids
  • Temperature

ASJC Scopus subject areas

  • Food Science
  • Analytical Chemistry

Cite this

Enzymatic synthesis of structured lipids using a novel cold-active lipase from Pichia lynferdii NRRL Y-7723. / Kim, Hak Ryul; Hou, Ching T.; Lee, Ki Teak; Kim, Byung Hee; Kim, In-Hwan.

In: Food Chemistry, Vol. 122, No. 3, 01.10.2010, p. 846-849.

Research output: Contribution to journalArticle

Kim, Hak Ryul ; Hou, Ching T. ; Lee, Ki Teak ; Kim, Byung Hee ; Kim, In-Hwan. / Enzymatic synthesis of structured lipids using a novel cold-active lipase from Pichia lynferdii NRRL Y-7723. In: Food Chemistry. 2010 ; Vol. 122, No. 3. pp. 846-849.
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