Enzyme-catalysed hydrolysis of phosphatidylcholine for the production of lysophosphatidylcholine

Ramiro Baeza-Jiménez, Leticia X. López-Martínez, Cristina Otero, In-Hwan Kim, Hugo S. García

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Background: Production of lysophosphatidylcholine (LPC) via enzyme-catalysed hydrolysis was studied. Three enzymes were employed to conduct the reactions at different temperatures. The starting material, phosphatidylcholine (PC), was dispersed in water (system A) and in ethanol (system B) to define the reaction mixture to carry out the trials. Results: It was found that the media employed and the type of biocatalyst (free or immobilized), clearly determine the kinetics of the reactions. PC was well dissolved in ethanol but an opaque emulsion was obtained when it was dissolved in water. Immobilized PLA1 and Novozym 435 were able to convert PC into LPC in ethanol, with yields of 50% and 58.51%, respectively, after 48h at 50°C. The highest degree of hydrolysis (70%) was reached with Lipase PS after 48h at 60°C in water. Conclusions: Both reaction media enabled fairly good yields but water was better. The present work proposes a simple reaction scheme compared with other reports in the literature where different substrates, additives and polar solvents have been employed. LPC has interesting properties as a bioemulsifier, which led us to develop new methods for its production.

Original languageEnglish
Pages (from-to)1859-1863
Number of pages5
JournalJournal of Chemical Technology and Biotechnology
Volume88
Issue number10
DOIs
Publication statusPublished - 2013 Oct 1

Fingerprint

Lysophosphatidylcholines
Phosphatidylcholines
ethanol
hydrolysis
Hydrolysis
Enzymes
enzyme
Ethanol
Water
water yield
emulsion
water
Biocatalysts
Lipases
Emulsions
Lipase
substrate
kinetics
Kinetics
Temperature

Keywords

  • Hydrolysis
  • Lipases
  • Lysophosphatidylcholine
  • Phosphatidylcholine
  • Phospholipase

ASJC Scopus subject areas

  • Inorganic Chemistry
  • Waste Management and Disposal
  • Pollution
  • Organic Chemistry
  • Renewable Energy, Sustainability and the Environment
  • Biotechnology
  • Chemical Engineering(all)
  • Fuel Technology

Cite this

Enzyme-catalysed hydrolysis of phosphatidylcholine for the production of lysophosphatidylcholine. / Baeza-Jiménez, Ramiro; López-Martínez, Leticia X.; Otero, Cristina; Kim, In-Hwan; García, Hugo S.

In: Journal of Chemical Technology and Biotechnology, Vol. 88, No. 10, 01.10.2013, p. 1859-1863.

Research output: Contribution to journalArticle

Baeza-Jiménez, Ramiro ; López-Martínez, Leticia X. ; Otero, Cristina ; Kim, In-Hwan ; García, Hugo S. / Enzyme-catalysed hydrolysis of phosphatidylcholine for the production of lysophosphatidylcholine. In: Journal of Chemical Technology and Biotechnology. 2013 ; Vol. 88, No. 10. pp. 1859-1863.
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