Enzyme-catalyzed resolution of racemate using enzyme functionalized silica nanoparticles in the presence of surfactants

Yoon Seok Song, Hee Uk Lee, Jong Ho Lee, Chulhwan Park, Seung Wook Kim

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The enzyme-catalyzed resolution of racemic naproxen 2,2,2-trifluoroethyl thioester was performed by the immobilization of lipase on silica nanoparticles using a covalent bonding method. To increase the conversion and reaction rate of this resolution, we investigated the effect of non-ionic surfactants (M-SA 1025 and SM 20). The optimal reaction conditions such as temperature and loading amount of immobilized lipase were also determined. The addition of M-SA 1025 resulted in the increase in reaction rate (VS), conversion (X S) and enantioselectivity (E value) comparison with SM 20 and the control. The reaction performed in a mixture containing M-SA 1025 at 50 °C with 80 U/mL of immobilized lipase markedly improved the resolution of racemic naproxen 2,2,2-trifluoroethyl thioester compared to other conditions.

Original languageEnglish
Pages (from-to)817-820
Number of pages4
JournalProcess Biochemistry
Volume46
Issue number3
DOIs
Publication statusPublished - 2011 Mar

Keywords

  • Immobilization
  • Lipase
  • Naproxen
  • Resolution
  • Silica nanoparticle
  • Surfactant

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

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