Enzyme-conjugated CdSe/ZnS quantum dot biosensors for glucose detection

Gang Il Kim, Yun Mo Sung

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Conjugated nanocrystals using CdSe/ZnS core/shell nanocrystal quantum dots modified by organic linkers and glucose oxidase (GOx) were prepared for use as biosensors. The trioctylphophine oxide (TOPO)-capped QDs were first modified to give them water-solubility by terminal carboxyl groups that were bonded to the amino groups of GOx through an EDC/NHS coupling reaction. As the glucose concentration increased, the photoluminescence intensity was enhanced linearly due to the electron transfer during the enzymatic reaction. The UV-visible spectra of the as-prepared QDs are identical to that of QDs-MAA. This shows that these QDs do not become agglomerated during ligand exchanges. A photoluminescence (PL) spectroscopic study showed that the PL intensity of the QDs-GOx bioconjugates was increased in the presence of glucose. These glucose sensors showed linearity up to approximately 15 mM and became gradually saturated above 15 mM because the excess glucose did not affect the enzymatic oxidation reaction past that amount. These biosensors show highly sensitive variation in terms of their photoluminescence depending on the glucose concentration.

Original languageEnglish
Pages (from-to)44-49
Number of pages6
JournalKorean Journal of Materials Research
Volume19
Issue number1
DOIs
Publication statusPublished - 2009 Aug 19

Fingerprint

Biosensors
Glucose Oxidase
Semiconductor quantum dots
Glucose oxidase
Glucose
Photoluminescence
Enzymes
Nanocrystals
Glucose sensors
Oxides
Solubility
Ligands
Oxidation
Electrons
Water

Keywords

  • CdSe/ZnS quantum dots
  • Glucose oxidase(GOx)
  • Ligand exchange
  • Photoluminescense(PL)

ASJC Scopus subject areas

  • Materials Science(all)

Cite this

Enzyme-conjugated CdSe/ZnS quantum dot biosensors for glucose detection. / Kim, Gang Il; Sung, Yun Mo.

In: Korean Journal of Materials Research, Vol. 19, No. 1, 19.08.2009, p. 44-49.

Research output: Contribution to journalArticle

@article{b6a690744edd4dbca4b13f7206955a73,
title = "Enzyme-conjugated CdSe/ZnS quantum dot biosensors for glucose detection",
abstract = "Conjugated nanocrystals using CdSe/ZnS core/shell nanocrystal quantum dots modified by organic linkers and glucose oxidase (GOx) were prepared for use as biosensors. The trioctylphophine oxide (TOPO)-capped QDs were first modified to give them water-solubility by terminal carboxyl groups that were bonded to the amino groups of GOx through an EDC/NHS coupling reaction. As the glucose concentration increased, the photoluminescence intensity was enhanced linearly due to the electron transfer during the enzymatic reaction. The UV-visible spectra of the as-prepared QDs are identical to that of QDs-MAA. This shows that these QDs do not become agglomerated during ligand exchanges. A photoluminescence (PL) spectroscopic study showed that the PL intensity of the QDs-GOx bioconjugates was increased in the presence of glucose. These glucose sensors showed linearity up to approximately 15 mM and became gradually saturated above 15 mM because the excess glucose did not affect the enzymatic oxidation reaction past that amount. These biosensors show highly sensitive variation in terms of their photoluminescence depending on the glucose concentration.",
keywords = "CdSe/ZnS quantum dots, Glucose oxidase(GOx), Ligand exchange, Photoluminescense(PL)",
author = "Kim, {Gang Il} and Sung, {Yun Mo}",
year = "2009",
month = "8",
day = "19",
doi = "10.3740/MRSK.2009.19.1.044",
language = "English",
volume = "19",
pages = "44--49",
journal = "Korean Journal of Materials Research",
issn = "1225-0562",
publisher = "The Korea Federation of Science and Technology",
number = "1",

}

TY - JOUR

T1 - Enzyme-conjugated CdSe/ZnS quantum dot biosensors for glucose detection

AU - Kim, Gang Il

AU - Sung, Yun Mo

PY - 2009/8/19

Y1 - 2009/8/19

N2 - Conjugated nanocrystals using CdSe/ZnS core/shell nanocrystal quantum dots modified by organic linkers and glucose oxidase (GOx) were prepared for use as biosensors. The trioctylphophine oxide (TOPO)-capped QDs were first modified to give them water-solubility by terminal carboxyl groups that were bonded to the amino groups of GOx through an EDC/NHS coupling reaction. As the glucose concentration increased, the photoluminescence intensity was enhanced linearly due to the electron transfer during the enzymatic reaction. The UV-visible spectra of the as-prepared QDs are identical to that of QDs-MAA. This shows that these QDs do not become agglomerated during ligand exchanges. A photoluminescence (PL) spectroscopic study showed that the PL intensity of the QDs-GOx bioconjugates was increased in the presence of glucose. These glucose sensors showed linearity up to approximately 15 mM and became gradually saturated above 15 mM because the excess glucose did not affect the enzymatic oxidation reaction past that amount. These biosensors show highly sensitive variation in terms of their photoluminescence depending on the glucose concentration.

AB - Conjugated nanocrystals using CdSe/ZnS core/shell nanocrystal quantum dots modified by organic linkers and glucose oxidase (GOx) were prepared for use as biosensors. The trioctylphophine oxide (TOPO)-capped QDs were first modified to give them water-solubility by terminal carboxyl groups that were bonded to the amino groups of GOx through an EDC/NHS coupling reaction. As the glucose concentration increased, the photoluminescence intensity was enhanced linearly due to the electron transfer during the enzymatic reaction. The UV-visible spectra of the as-prepared QDs are identical to that of QDs-MAA. This shows that these QDs do not become agglomerated during ligand exchanges. A photoluminescence (PL) spectroscopic study showed that the PL intensity of the QDs-GOx bioconjugates was increased in the presence of glucose. These glucose sensors showed linearity up to approximately 15 mM and became gradually saturated above 15 mM because the excess glucose did not affect the enzymatic oxidation reaction past that amount. These biosensors show highly sensitive variation in terms of their photoluminescence depending on the glucose concentration.

KW - CdSe/ZnS quantum dots

KW - Glucose oxidase(GOx)

KW - Ligand exchange

KW - Photoluminescense(PL)

UR - http://www.scopus.com/inward/record.url?scp=68649099014&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=68649099014&partnerID=8YFLogxK

U2 - 10.3740/MRSK.2009.19.1.044

DO - 10.3740/MRSK.2009.19.1.044

M3 - Article

AN - SCOPUS:68649099014

VL - 19

SP - 44

EP - 49

JO - Korean Journal of Materials Research

JF - Korean Journal of Materials Research

SN - 1225-0562

IS - 1

ER -