Enzyme-conjugated ZnO nanocrystals for collisional quenching-based glucose sensing

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

A simple approach to sensitive glucose detection has been developed based upon variation in the fluorescence of ZnO nanocrystals with glucose concentration. ZnO nanocrystals were successfully synthesized in wurtzite structure using a surfactant, mercaptoundecanoic acid (MUA) via the polyol method. MUA molecules not only served as a template for the synthesis of spherical-shape nanoparticles but also provided water solubility and biocompatibility due to its carboxyl group. Carboxyl-terminated ZnO nanocrystals were activated by esterification of n-hydroxysulfosuccinimide (Sulfo-NHS) catalyzed by water-soluble 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC). Glucose oxidase (GOx), an enzyme could be immobilized to ZnO nanocrystals by replacing NHS with amino-acid groups of GOx. ZnO-MUA-GOx bioconjugates showed a decrease in the photoluminescence (PL) intensity by appearance of glucose molecules due to the collisional quenching by hydrogen peroxide generated from enzymatic oxidation reaction of glucose. PL intensity showed a linear decrease with glucose concentration from 1.6 to 33.3 mM, which fully covers the physiological glucose level. ZnO-MUA-GOx bioconjugates showed a detection limit lower than 0.33 mM and a response time less than 5 s. They also revealed distinct specificity against cholesterol molecules.

Original languageEnglish
Pages (from-to)2859-2865
Number of pages7
JournalCrystEngComm
Volume14
Issue number8
DOIs
Publication statusPublished - 2012 Apr 21

Fingerprint

glucose
Nanocrystals
Glucose
enzymes
Quenching
Glucose Oxidase
nanocrystals
Glucose oxidase
Enzymes
quenching
oxidase
Acids
Molecules
Photoluminescence
Ethyldimethylaminopropyl Carbodiimide
acids
Water
Polyols
Cholesterol
Esterification

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics

Cite this

Enzyme-conjugated ZnO nanocrystals for collisional quenching-based glucose sensing. / Kim, Ki Eun; Kim, Tae Geun; Sung, Yun Mo.

In: CrystEngComm, Vol. 14, No. 8, 21.04.2012, p. 2859-2865.

Research output: Contribution to journalArticle

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