Enzyme-Immobilized Chitosan Nanoparticles as Environmentally Friendly and Highly Effective Antimicrobial Agents

Kyung Min Yeon, Jisung You, Manab Deb Adhikari, Sung Gil Hong, Inseon Lee, Han Sol Kim, Li Na Kim, Jahyun Nam, Seok Joon Kwon, Moon Il Kim, Warayuth Sajomsang, Jonathan S. Dordick, Jungbae Kim

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Highly effective and minimally toxic antimicrobial agents have been prepared by immobilizing glucose oxidase (GOx) onto biocompatible chitosan nanoparticles (CS-NPs). CS-NPs were prepared via ionotropic gelation and used for the immobilization of GOx via approaches of covalent attachment (CA), enzyme coating (EC), enzyme precipitate coating (EPC), and magnetic nanoparticle-incorporated EPC (Mag-EPC). EPC represents an approach consisting of enzyme covalent attachment, precipitation, and cross-linking, with CA and EC being control samples while Mag-EPC was prepared by mixing magnetic nanoparticles (Mag) with enzymes during the preparation of EPC. The GOx activities of CA, EC, EPC, and Mag-EPC were 8.57, 17.7, 219, and 247 units/mg CS-NPs, respectively, representing 26 and 12 times higher activity of EPC than those of CA and EC, respectively. EPC improved the activity and stability of GOx and led to good dispersion of CS-NPs, while Mag-EPC enabled facile magnetic separation. To demonstrate the expandability of the EPC approach to other enzymes, bovine carbonic anhydrase was also employed to prepare EPC and Mag-EPC samples for their characterizations. In the presence of glucose, EPC of GOx generated H2O2 in situ, which effectively inhibited the proliferation of Staphylococcus aureus in both suspended cultures and biofilms, thereby demonstrating the potential of EPC-GOx as environmentally friendly and highly effective antimicrobial materials.

Original languageEnglish
Pages (from-to)2477-2485
Number of pages9
JournalBiomacromolecules
Volume20
Issue number7
DOIs
Publication statusPublished - 2019 Jul 8

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Antimicrobial agents
Immobilized Enzymes
Chitosan
Anti-Infective Agents
Enzymes
Nanoparticles
Precipitates
Coatings
Glucose Oxidase
Glucose oxidase
Catalyst activity
Ionotropic gelation
Carbonic anhydrase
Magnetic separation

ASJC Scopus subject areas

  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

Cite this

Enzyme-Immobilized Chitosan Nanoparticles as Environmentally Friendly and Highly Effective Antimicrobial Agents. / Yeon, Kyung Min; You, Jisung; Adhikari, Manab Deb; Hong, Sung Gil; Lee, Inseon; Kim, Han Sol; Kim, Li Na; Nam, Jahyun; Kwon, Seok Joon; Kim, Moon Il; Sajomsang, Warayuth; Dordick, Jonathan S.; Kim, Jungbae.

In: Biomacromolecules, Vol. 20, No. 7, 08.07.2019, p. 2477-2485.

Research output: Contribution to journalArticle

Yeon, KM, You, J, Adhikari, MD, Hong, SG, Lee, I, Kim, HS, Kim, LN, Nam, J, Kwon, SJ, Kim, MI, Sajomsang, W, Dordick, JS & Kim, J 2019, 'Enzyme-Immobilized Chitosan Nanoparticles as Environmentally Friendly and Highly Effective Antimicrobial Agents', Biomacromolecules, vol. 20, no. 7, pp. 2477-2485. https://doi.org/10.1021/acs.biomac.9b00152
Yeon, Kyung Min ; You, Jisung ; Adhikari, Manab Deb ; Hong, Sung Gil ; Lee, Inseon ; Kim, Han Sol ; Kim, Li Na ; Nam, Jahyun ; Kwon, Seok Joon ; Kim, Moon Il ; Sajomsang, Warayuth ; Dordick, Jonathan S. ; Kim, Jungbae. / Enzyme-Immobilized Chitosan Nanoparticles as Environmentally Friendly and Highly Effective Antimicrobial Agents. In: Biomacromolecules. 2019 ; Vol. 20, No. 7. pp. 2477-2485.
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