Enzyme loaded alginate hollow fiber for reactor

Kwang Ho Lee, Amit Asthana, Su Jung Shin, Sang Hoon Lee, Dong Pyo Kim

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this paper, we present the fabrication of an enzyme loaded alginate hollow fiber synthesize using a polydimethelysiloxane (PDMS)-based microfluidic chip. This is a novel bio-catalytic micro-chemical reactor. Vanadium containing bromoperoxidase (VBPO) enzyme isolated from Corallina Confusa was used as model enzyme to perform bromo-oxidation of phenol red to bromophenol blue at different flow rates in synthesized alginate hollow fiber. We have shown an easy approach to fabricate a microfluidic bio-catalytic reactor. We have also demonstrated about 5.8 time faster reaction kinetic with this kind of microfluidic bio-reactor.

Original languageEnglish
Title of host publicationProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages645-647
Number of pages3
ISBN (Print)9780979806421
Publication statusPublished - 2009 Jan 1
Event13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 - Jeju, Korea, Republic of
Duration: 2009 Nov 12009 Nov 5

Publication series

NameProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Other

Other13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
CountryKorea, Republic of
CityJeju
Period09/11/109/11/5

Keywords

  • Alginate hollow fiber
  • Bio-reactor
  • Bromo-oxidation
  • Enzyme

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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  • Cite this

    Lee, K. H., Asthana, A., Shin, S. J., Lee, S. H., & Kim, D. P. (2009). Enzyme loaded alginate hollow fiber for reactor. In Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 645-647). (Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences). Chemical and Biological Microsystems Society.