Abstract
In this paper, we present the fabrication of an enzyme loaded alginate hollow fiber synthesize using a polydimethelysiloxane (PDMS)-based microfluidic chip. This is a novel bio-catalytic micro-chemical reactor. Vanadium containing bromoperoxidase (VBPO) enzyme isolated from Corallina Confusa was used as model enzyme to perform bromo-oxidation of phenol red to bromophenol blue at different flow rates in synthesized alginate hollow fiber. We have shown an easy approach to fabricate a microfluidic bio-catalytic reactor. We have also demonstrated about 5.8 time faster reaction kinetic with this kind of microfluidic bio-reactor.
| Original language | English |
|---|---|
| Title of host publication | Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 645-647 |
| Number of pages | 3 |
| ISBN (Print) | 9780979806421 |
| Publication status | Published - 2009 Jan 1 |
| Event | 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 - Jeju, Korea, Republic of Duration: 2009 Nov 1 → 2009 Nov 5 |
Other
| Other | 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 |
|---|---|
| Country | Korea, Republic of |
| City | Jeju |
| Period | 09/11/1 → 09/11/5 |
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ASJC Scopus subject areas
- Chemical Engineering (miscellaneous)
- Bioengineering
Cite this
Enzyme loaded alginate hollow fiber for reactor. / Lee, Kwang Ho; Asthana, Amit; Shin, Su Jung; Lee, Sang Hoon; Kim, Dong Pyo.
Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2009. p. 645-647.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Enzyme loaded alginate hollow fiber for reactor
AU - Lee, Kwang Ho
AU - Asthana, Amit
AU - Shin, Su Jung
AU - Lee, Sang Hoon
AU - Kim, Dong Pyo
PY - 2009/1/1
Y1 - 2009/1/1
N2 - In this paper, we present the fabrication of an enzyme loaded alginate hollow fiber synthesize using a polydimethelysiloxane (PDMS)-based microfluidic chip. This is a novel bio-catalytic micro-chemical reactor. Vanadium containing bromoperoxidase (VBPO) enzyme isolated from Corallina Confusa was used as model enzyme to perform bromo-oxidation of phenol red to bromophenol blue at different flow rates in synthesized alginate hollow fiber. We have shown an easy approach to fabricate a microfluidic bio-catalytic reactor. We have also demonstrated about 5.8 time faster reaction kinetic with this kind of microfluidic bio-reactor.
AB - In this paper, we present the fabrication of an enzyme loaded alginate hollow fiber synthesize using a polydimethelysiloxane (PDMS)-based microfluidic chip. This is a novel bio-catalytic micro-chemical reactor. Vanadium containing bromoperoxidase (VBPO) enzyme isolated from Corallina Confusa was used as model enzyme to perform bromo-oxidation of phenol red to bromophenol blue at different flow rates in synthesized alginate hollow fiber. We have shown an easy approach to fabricate a microfluidic bio-catalytic reactor. We have also demonstrated about 5.8 time faster reaction kinetic with this kind of microfluidic bio-reactor.
KW - Alginate hollow fiber
KW - Bio-reactor
KW - Bromo-oxidation
KW - Enzyme
UR - http://www.scopus.com/inward/record.url?scp=84901783953&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84901783953&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84901783953
SN - 9780979806421
SP - 645
EP - 647
BT - Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
ER -