Abstract
In this paper, we present the fabrication of an enzyme loaded alginate hollow fiber synthesize using a polydimethelysiloxane (PDMS)-based microfluidic chip. This is a novel bio-catalytic micro-chemical reactor. Vanadium containing bromoperoxidase (VBPO) enzyme isolated from Corallina Confusa was used as model enzyme to perform bromo-oxidation of phenol red to bromophenol blue at different flow rates in synthesized alginate hollow fiber. We have shown an easy approach to fabricate a microfluidic bio-catalytic reactor. We have also demonstrated about 5.8 time faster reaction kinetic with this kind of microfluidic bio-reactor.
| Original language | English |
|---|---|
| Title of host publication | Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 645-647 |
| Number of pages | 3 |
| ISBN (Print) | 9780979806421 |
| Publication status | Published - 2009 Jan 1 |
| Event | 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 - Jeju, Korea, Republic of Duration: 2009 Nov 1 → 2009 Nov 5 |
Publication series
| Name | Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
|---|
Other
| Other | 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 |
|---|---|
| Country | Korea, Republic of |
| City | Jeju |
| Period | 09/11/1 → 09/11/5 |
Keywords
- Alginate hollow fiber
- Bio-reactor
- Bromo-oxidation
- Enzyme
ASJC Scopus subject areas
- Chemical Engineering (miscellaneous)
- Bioengineering
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Cite this
Lee, K. H., Asthana, A., Shin, S. J., Lee, S. H., & Kim, D. P. (2009). Enzyme loaded alginate hollow fiber for reactor. In Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 645-647). (Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences). Chemical and Biological Microsystems Society.