Enzyme logic gates based on enzyme-coated carbon nanotubes

Kyung Woo Kim, Byung Chan Kim, Hye Jin Lee, Jungbae Kim, Min-Kyu Oh

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Enzyme-based Boolean logic gates were developed by using highly stable enzyme precipitate coatings (EPCs) on carbon nanotubes (CNTs). EPCs of three enzymes, glucose oxidase (GOx), horseradish peroxidase (HRP), and invertase (INV), were prepared in a three-step process that consists of covalent enzyme attachment, enzyme precipitation, and enzyme crosslinking. The AND logic gate was formulated based on an ON output signal in response to the combined inputs of glucose and dye. The OR logic gate was based on the ON output signal of glucose oxidation in the presence of EPC-GOx and EPC-INV with one of the two inputs, either glucose or sucrose. The use of EPCs will provide an effective tool for the development of sensitive and repeatedly-usable Boolean logic gates with a long lifetime, which can be employed in various biosignaling systems.

Original languageEnglish
Pages (from-to)980-986
Number of pages7
JournalElectroanalysis
Volume23
Issue number4
DOIs
Publication statusPublished - 2011 Apr 1

Fingerprint

Carbon Nanotubes
Logic gates
Carbon nanotubes
Enzymes
Precipitates
Coatings
Glucose
beta-Fructofuranosidase
Glucose Oxidase
Glucose oxidase
Sugar (sucrose)
Horseradish Peroxidase
Crosslinking
Sucrose
Coloring Agents
Dyes
Oxidation

Keywords

  • Boolean logic gates
  • Carbon nanotubes
  • Enzyme precipitate coatings
  • Glucose oxidase
  • Horseradish peroxidase
  • Invertase

ASJC Scopus subject areas

  • Analytical Chemistry
  • Electrochemistry

Cite this

Enzyme logic gates based on enzyme-coated carbon nanotubes. / Kim, Kyung Woo; Kim, Byung Chan; Lee, Hye Jin; Kim, Jungbae; Oh, Min-Kyu.

In: Electroanalysis, Vol. 23, No. 4, 01.04.2011, p. 980-986.

Research output: Contribution to journalArticle

Kim, Kyung Woo ; Kim, Byung Chan ; Lee, Hye Jin ; Kim, Jungbae ; Oh, Min-Kyu. / Enzyme logic gates based on enzyme-coated carbon nanotubes. In: Electroanalysis. 2011 ; Vol. 23, No. 4. pp. 980-986.
@article{d4b7f8b695054172a6df3599c3152087,
title = "Enzyme logic gates based on enzyme-coated carbon nanotubes",
abstract = "Enzyme-based Boolean logic gates were developed by using highly stable enzyme precipitate coatings (EPCs) on carbon nanotubes (CNTs). EPCs of three enzymes, glucose oxidase (GOx), horseradish peroxidase (HRP), and invertase (INV), were prepared in a three-step process that consists of covalent enzyme attachment, enzyme precipitation, and enzyme crosslinking. The AND logic gate was formulated based on an ON output signal in response to the combined inputs of glucose and dye. The OR logic gate was based on the ON output signal of glucose oxidation in the presence of EPC-GOx and EPC-INV with one of the two inputs, either glucose or sucrose. The use of EPCs will provide an effective tool for the development of sensitive and repeatedly-usable Boolean logic gates with a long lifetime, which can be employed in various biosignaling systems.",
keywords = "Boolean logic gates, Carbon nanotubes, Enzyme precipitate coatings, Glucose oxidase, Horseradish peroxidase, Invertase",
author = "Kim, {Kyung Woo} and Kim, {Byung Chan} and Lee, {Hye Jin} and Jungbae Kim and Min-Kyu Oh",
year = "2011",
month = "4",
day = "1",
doi = "10.1002/elan.201000634",
language = "English",
volume = "23",
pages = "980--986",
journal = "Electroanalysis",
issn = "1040-0397",
publisher = "Wiley-VCH Verlag",
number = "4",

}

TY - JOUR

T1 - Enzyme logic gates based on enzyme-coated carbon nanotubes

AU - Kim, Kyung Woo

AU - Kim, Byung Chan

AU - Lee, Hye Jin

AU - Kim, Jungbae

AU - Oh, Min-Kyu

PY - 2011/4/1

Y1 - 2011/4/1

N2 - Enzyme-based Boolean logic gates were developed by using highly stable enzyme precipitate coatings (EPCs) on carbon nanotubes (CNTs). EPCs of three enzymes, glucose oxidase (GOx), horseradish peroxidase (HRP), and invertase (INV), were prepared in a three-step process that consists of covalent enzyme attachment, enzyme precipitation, and enzyme crosslinking. The AND logic gate was formulated based on an ON output signal in response to the combined inputs of glucose and dye. The OR logic gate was based on the ON output signal of glucose oxidation in the presence of EPC-GOx and EPC-INV with one of the two inputs, either glucose or sucrose. The use of EPCs will provide an effective tool for the development of sensitive and repeatedly-usable Boolean logic gates with a long lifetime, which can be employed in various biosignaling systems.

AB - Enzyme-based Boolean logic gates were developed by using highly stable enzyme precipitate coatings (EPCs) on carbon nanotubes (CNTs). EPCs of three enzymes, glucose oxidase (GOx), horseradish peroxidase (HRP), and invertase (INV), were prepared in a three-step process that consists of covalent enzyme attachment, enzyme precipitation, and enzyme crosslinking. The AND logic gate was formulated based on an ON output signal in response to the combined inputs of glucose and dye. The OR logic gate was based on the ON output signal of glucose oxidation in the presence of EPC-GOx and EPC-INV with one of the two inputs, either glucose or sucrose. The use of EPCs will provide an effective tool for the development of sensitive and repeatedly-usable Boolean logic gates with a long lifetime, which can be employed in various biosignaling systems.

KW - Boolean logic gates

KW - Carbon nanotubes

KW - Enzyme precipitate coatings

KW - Glucose oxidase

KW - Horseradish peroxidase

KW - Invertase

UR - http://www.scopus.com/inward/record.url?scp=79953267399&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79953267399&partnerID=8YFLogxK

U2 - 10.1002/elan.201000634

DO - 10.1002/elan.201000634

M3 - Article

AN - SCOPUS:79953267399

VL - 23

SP - 980

EP - 986

JO - Electroanalysis

JF - Electroanalysis

SN - 1040-0397

IS - 4

ER -