Enzyme precipitate coatings of glucose oxidase onto carbon paper for biofuel cell applications

Mike Fischback, Ki Young Kwon, Inseon Lee, Su Jeong Shin, Hyun Gyu Park, Byoung Chan Kim, Yongchai Kwon, Hee Tae Jung, Jungbae Kim, Su Ha

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Enzymatic biofuel cells (BFC) have a great potential as a small power source, but their practical applications are being hampered by short lifetime and low power density. This study describes the direct immobilization of glucose oxidase (GOx) onto the carbon paper in the form of highly stable and active enzyme precipitation coatings (EPCs), which can improve the lifetime and power density of BFCs. EPCs were fabricated directly onto the carbon paper via a three-step process: covalent attachment (CA), enzyme precipitation, and chemical crosslinking. GOx-immobilized carbon papers via the CA and EPC approaches were used as an enzyme anode and their electrochemical activities were tested under the BFC-operating mode. The BFCs with CA and EPC enzyme anodes produced the maximum power densities of 50 and 250μW/cm 2, respectively. The BFC with the EPC enzyme anode showed a stable current density output of >700μA/cm 2 at 0.18V under continuous operation for over 45h. When a maple syrup was used as a fuel under ambient conditions, it also produced a stable current density of >10μA/cm 2 at 0.18V for over 25h. It is anticipated that the direct immobilization of EPC on hierarchical-structured electrodes with a large surface area would further improve the power density of BFCs that can make their applications more feasible.

Original languageEnglish
Pages (from-to)318-324
Number of pages7
JournalBiotechnology and Bioengineering
Volume109
Issue number2
DOIs
Publication statusPublished - 2012 Feb 1

Fingerprint

Bioelectric Energy Sources
Biological fuel cells
Glucose Oxidase
Glucose oxidase
Precipitates
Carbon
Enzymes
Coatings
Electrodes
Anodes
Immobilization
Enzymatic fuel cells
Chemical Precipitation
Current density
Acer
Electric Power Supplies
Crosslinking

Keywords

  • Biofuel cell
  • Carbon paper
  • Enzyme precipitation and coating
  • Glucose oxidase
  • Maple syrup

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Fischback, M., Kwon, K. Y., Lee, I., Shin, S. J., Park, H. G., Kim, B. C., ... Ha, S. (2012). Enzyme precipitate coatings of glucose oxidase onto carbon paper for biofuel cell applications. Biotechnology and Bioengineering, 109(2), 318-324. https://doi.org/10.1002/bit.23317

Enzyme precipitate coatings of glucose oxidase onto carbon paper for biofuel cell applications. / Fischback, Mike; Kwon, Ki Young; Lee, Inseon; Shin, Su Jeong; Park, Hyun Gyu; Kim, Byoung Chan; Kwon, Yongchai; Jung, Hee Tae; Kim, Jungbae; Ha, Su.

In: Biotechnology and Bioengineering, Vol. 109, No. 2, 01.02.2012, p. 318-324.

Research output: Contribution to journalArticle

Fischback, M, Kwon, KY, Lee, I, Shin, SJ, Park, HG, Kim, BC, Kwon, Y, Jung, HT, Kim, J & Ha, S 2012, 'Enzyme precipitate coatings of glucose oxidase onto carbon paper for biofuel cell applications', Biotechnology and Bioengineering, vol. 109, no. 2, pp. 318-324. https://doi.org/10.1002/bit.23317
Fischback, Mike ; Kwon, Ki Young ; Lee, Inseon ; Shin, Su Jeong ; Park, Hyun Gyu ; Kim, Byoung Chan ; Kwon, Yongchai ; Jung, Hee Tae ; Kim, Jungbae ; Ha, Su. / Enzyme precipitate coatings of glucose oxidase onto carbon paper for biofuel cell applications. In: Biotechnology and Bioengineering. 2012 ; Vol. 109, No. 2. pp. 318-324.
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