Enzyme precipitate coatings of lipase on polymer nanofibers

Hyo Jin An, Hye Jin Lee, Seung Hyun Jun, Sang Youn Hwang, Byoung Chan Kim, Kwanghee Kim, Kyung Mi Lee, Min Kyu Oh, Jungbae Kim

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Lipase (LP) was immobilized on electrospun and ethanol-dispersed polystyrene-poly(styrene-co-maleic anhydride) (PS-PSMA) nanofibers (EtOH-NF) in the form of enzyme precipitate coatings (EPCs). LP precipitate coatings (EPCs-LP) were prepared in a three-step process, consisting of covalent attachment, LP precipitation, and crosslinking of precipitated LPs onto the covalently attached LPs via glutaraldehyde treatment. The LP precipitation was performed by adding various concentrations of ammonium sulfate (20-50%, w/v). EPCs-LP improved the LP activity and stability when compared to covalently attached LPs (CA-LP) and the enzyme coatings of LPs (EC-LP) without the LP precipitation. For example, the use of 40% (w/v) ammonium sulfate resulted in EPC40-LP with the highest activity, which was 4.0 and 3.6 times higher than those of CA-LP and EC-LP, respectively. After 165-day incubation under rigorous shaking at 200 rpm, the residual activities of EPC50-LP were 0.5 lM/min mg of EtOH-NF, representing 113 and 75 times higher than those of CA-LP and EC-LP, respectively. When LP was partially purified via a simple ammonium sulfate precipitation and dialysis, both activities and stabilities of EC-LP and EPCLP could be marginally improved. It is anticipated that the improved LP activity and stability in the form of EPCs would allow for their potential applications in various bioconversion processes such as biodiesel production and ibuprofen resolution.

Original languageEnglish
Pages (from-to)841-847
Number of pages7
JournalBioprocess and Biosystems Engineering
Volume34
Issue number7
DOIs
Publication statusPublished - 2011 Sep

Keywords

  • Electrospun polymer nanofibers
  • Enzyme precipitate coatings
  • Enzyme stabilization
  • Ethanol dispersion
  • Lipase

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering

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