Eotaxin induces migration of RBL-2H3 mast cells via a Rac-ERK-dependent pathway

Chang Hoon Woo, Dong Tak Jeong, Seog Beom Yoon, Key Sun Kim, Il Yup Chung, Toshihiko Saeki, Jae Hong Kim

Research output: Contribution to journalArticlepeer-review

35 Citations (Scopus)

Abstract

Eotaxin is a potent chemokine that acts via CC chemokine receptor 3 (CCR3)to induce chemotaxis, mainly on eosinophils. Here we show that eotaxin also induces chemotactic migration in rat basophilic leukemia (RBL-2H3) mast cells. This effect was dose-dependently inhibited by compound X, a selective CCR3 antagonist, indicating that, as in eosinophils, the effect was mediated by CCR3. Eotaxin-induced cell migration was completely blocked in RBL-RacN17 cells expressing adominant negative Rac1 mutant, suggesting a crucial role for Rac1 in eotaxin signaling to chemotactic migration. ERK activation also proved essential for eotaxin signaling and it too was absent in RBL-RacN17 cells. Finally, we found that activation of Rac and ERK was correlated with eotaxin-induced actin reorganization known to be necessary for cell motility. It thus appears that Rac1 acts upstream of ERK to signal chemotaxis in these cells, and that a Rac-ERK-dependent cascade mediates the eotaxin-induced chemotactic motility of RBL-2H3 mast cells.

Original languageEnglish
Pages (from-to)392-397
Number of pages6
JournalBiochemical and biophysical research communications
Volume298
Issue number3
DOIs
Publication statusPublished - 2002

Keywords

  • Chemotaxis
  • ERK
  • Eotaxin
  • RBL-2H3
  • Rac

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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