Eotaxin mRNA expression in bronchial mucosa of patients with asthma

He In Kwang He In, Yun Cho Jae Yun Cho, Yong Kang Sae Yong Kang, Youb Lee Sang Youb Lee, Jeong Shim Jae Jeong Shim, Ho Kang Kyung Ho Kang, Hwa Yoo Se Hwa Yoo, Soon Na Young Soon Na, Gyum Kim Han Gyum Kim

Research output: Contribution to journalArticle

Abstract

Background: Asthma is a chronic inflammatory disease of the airways characterized by a marked infiltration of eosinophils in the bronchial mucosa. Asthmatic bronchial mucosa produces many factors described as being chemotactic for inflammatory cells. IL-5, RANTES, and MCP-1 alpha are the chemotactic factors for eosinophils, but their roles are controversial. Recently eotaxin that is a potent eosinophil chemoattractant cytokine was detected in a guinea-pig model of allergic airway inflammation, and human eotaxin was cloned. Eotaxin is a specific chemoattractant for eosinophils, but its role in asthma is not confirmed. We examined the in vivo expression of eotaxin in bronchi of asthmatic patients. Methods: 11 asthmatics and 2 normal controls were enrolled. All subjects were underwent bronchoscopy with bronchial biopsies in 2nd or 3rd carina. RNA extraction from biopsy samples was done by acid-guanidium method. Semi-quantitative RT-PCR was done for evaluation of eotaxin mRNA expression. The extent of eosinophil infiltration was evaluated by counting the eosinophils in submucosa in HPF of microscope. Results: Eotaxin mRNA expressed in symptomatic, uncontrolled asthma. Steroid inhibited expression of eotaxin mRNA in asthma. Expression of eotaxin mRNA correlated with eosinophil infiltration in bronchial tissues. Conclusion: Expression of eotaxin mRNA increases in uncontrolled asthma and eotaxin is involved in the recruitment of eosinophils.

Original languageEnglish
Pages (from-to)697-704
Number of pages8
JournalTuberculosis and Respiratory Diseases
Volume45
Issue number4
Publication statusPublished - 1998

Keywords

  • Asthma
  • Bronchial mucosa
  • Eotaxin

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Infectious Diseases

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  • Cite this

    Kwang He In, H. I., Jae Yun Cho, Y. C., Sae Yong Kang, Y. K., Sang Youb Lee, Y. L., Jae Jeong Shim, J. S., Kyung Ho Kang, H. K., Se Hwa Yoo, H. Y., Young Soon Na, S. N., & Han Gyum Kim, G. K. (1998). Eotaxin mRNA expression in bronchial mucosa of patients with asthma. Tuberculosis and Respiratory Diseases, 45(4), 697-704.