(-)-Epicatechin gallate (ECG) stimulates osteoblast differentiation via runt-related transcription factor 2 (RUNX2) and transcriptional coactivator with PDZ-binding motif (TAZ)-mediated transcriptional activation

Mi Ran Byun, Mi Kyung Sung, A. Rum Kim, Cham Han Lee, Eun Jung Jang, Mi Gyeong Jeong, Minsoo Noh, Eun Sook Hwang, Jeong-Ho Hong

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Abstract

Osteoporosis is a degenerative bone disease characterized by low bone mass and is caused by an imbalance between osteoblastic bone formation and osteoclastic bone resorption. It is known that the bioactive compounds present in green tea increase osteogenic activity and decrease the risk of fracture by improving bone mineral density. However, the detailed mechanism underlying these beneficial effects has yet to be elucidated. In this study, we investigated the osteogenic effect of (-)-epicatechin gallate (ECG), a major bioactive compound found in green tea. We found that ECG effectively stimulates osteoblast differentiation, indicated by the increased expression of osteoblastic marker genes. Up-regulation of osteoblast marker genes is mediated by increased expression and interaction of the transcriptional coactivator with PDZ-binding motif (TAZ) and Runt-related transcription factor 2 (RUNX2). ECG facilitates nuclear localization ofTAZthrough PP1A. PP1A is essential for osteoblast differentiation because inhibition of PP1A activity was shown to suppress ECG-mediated osteogenic differentiation. Taken together, the results showed that ECG stimulates osteoblast differentiation through the activation of TAZ and RUNX2, revealing a novel mechanism for green tea-stimulated osteoblast differentiation.

Original languageEnglish
Pages (from-to)9926-9935
Number of pages10
JournalJournal of Biological Chemistry
Volume289
Issue number14
DOIs
Publication statusPublished - 2014 Apr 4

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Osteoblasts
Transcriptional Activation
Bone
Transcription Factors
Chemical activation
Tea
Genes
Bone Diseases
Bone Resorption
Osteogenesis
Bone Density
Osteoporosis
Minerals
Up-Regulation
epicatechin gallate
Bone and Bones

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

(-)-Epicatechin gallate (ECG) stimulates osteoblast differentiation via runt-related transcription factor 2 (RUNX2) and transcriptional coactivator with PDZ-binding motif (TAZ)-mediated transcriptional activation. / Byun, Mi Ran; Sung, Mi Kyung; Kim, A. Rum; Lee, Cham Han; Jang, Eun Jung; Jeong, Mi Gyeong; Noh, Minsoo; Hwang, Eun Sook; Hong, Jeong-Ho.

In: Journal of Biological Chemistry, Vol. 289, No. 14, 04.04.2014, p. 9926-9935.

Research output: Contribution to journalArticle

Byun, Mi Ran ; Sung, Mi Kyung ; Kim, A. Rum ; Lee, Cham Han ; Jang, Eun Jung ; Jeong, Mi Gyeong ; Noh, Minsoo ; Hwang, Eun Sook ; Hong, Jeong-Ho. / (-)-Epicatechin gallate (ECG) stimulates osteoblast differentiation via runt-related transcription factor 2 (RUNX2) and transcriptional coactivator with PDZ-binding motif (TAZ)-mediated transcriptional activation. In: Journal of Biological Chemistry. 2014 ; Vol. 289, No. 14. pp. 9926-9935.
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