Epidermal growth factor increases insulin secretion and lowers blood glucose in diabetic mice

H. Y. Lee, K. Yea, J. Kim, B. D. Lee, Y. C. Chae, H. S. Kim, D. W. Lee, S. H. Kim, J. H. Cho, C. J. Jin, D. S. Koh, K. S. Park, P. G. Suh, S. H. Ryu

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22 Citations (Scopus)

Abstract

Epidermal growth factor (EGF) is synthesized in the pancreas and diabetic animals have low levels of EGF. However, the role of EGF in regulating the major function of the pancreas, insulin secretion, has not been studied. Here, we show that EGF rapidly increased insulin secretion in mouse pancreatic islets, as well as in a pancreatic β-cell line. These events were dependent on a Ca2+ influx and phospholipase D (PLD) activity, particularly PLD2, as determined using pharmacological blockers and molecular manipulations such as over-expression and siRNA of PLD isozymes. In addition, EGF also increased plasma insulin levels and mediated glucose lowering in normal and diabetic mice. Here, for the first time, we provide evidence that EGF is a novel secretagogue that regulates plasma glucose levels and a candidate for the development of therapeutics for diabetes.

Original languageEnglish
Pages (from-to)1593-1604
Number of pages12
JournalJournal of Cellular and Molecular Medicine
Volume12
Issue number5A
DOIs
Publication statusPublished - 2008 Sep

Keywords

  • Epidermal growth factor
  • Glucose homeostasis
  • Insulin secretion
  • Phospholipase D2

ASJC Scopus subject areas

  • Molecular Medicine
  • Cell Biology

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    Lee, H. Y., Yea, K., Kim, J., Lee, B. D., Chae, Y. C., Kim, H. S., Lee, D. W., Kim, S. H., Cho, J. H., Jin, C. J., Koh, D. S., Park, K. S., Suh, P. G., & Ryu, S. H. (2008). Epidermal growth factor increases insulin secretion and lowers blood glucose in diabetic mice. Journal of Cellular and Molecular Medicine, 12(5A), 1593-1604. https://doi.org/10.1111/j.1582-4934.2007.00169.x