(-)-Epigallocatechin-3-gallate stimulates myogenic differentiation through TAZ activation

A. Rum Kim, Kyung Min Kim, Mi Ran Byun, Jun Ha Hwang, Jung Il Park, Ho Taek Oh, Mi Gyeong Jeong, Eun Sook Hwang, Jeong-Ho Hong

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Muscle loss is a typical process of aging. Green tea consumption is known to slow down the progress of aging. Their underlying mechanisms, however, remain largely unknown. In this study, we investigated the effect of (-)-epigallocatechin-3-gallate (EGCG), a polyphenolic compound of green tea, on myogenic differentiation and found that EGCG significantly increases myogenic differentiation. After EGCG treatment, the expression of myogenic marker genes, such as myosin heavy chain, are increased through activation of TAZ, a transcriptional coactivator with a PDZ-binding motif. TAZ-knockdown does not stimulate EGCG-induced myogenic differentiation. EGCG facilitates the interaction between TAZ and MyoD, which stimulates MyoD-mediated gene transcription. EGCG induces nuclear localization of TAZ through the dephosphorylation of TAZ at its Ser89 residue, which relieves 14-3-3 binding in the cytosol. Interestingly, inactivation of Lats kinase is observed after EGCG treatment, which is responsible for the production of dephosphorylated TAZ. Together, these results suggest that EGCG induces myogenic differentiation through TAZ, suggesting that TAZ plays an important role in EGCG induced muscle regeneration.

Original languageEnglish
Pages (from-to)378-384
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume486
Issue number2
DOIs
Publication statusPublished - 2017 Apr 29

Keywords

  • Catechins
  • Myogenesis
  • Satellite cells
  • TAZ

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Kim, A. R., Kim, K. M., Byun, M. R., Hwang, J. H., Park, J. I., Oh, H. T., Jeong, M. G., Hwang, E. S., & Hong, J-H. (2017). (-)-Epigallocatechin-3-gallate stimulates myogenic differentiation through TAZ activation. Biochemical and Biophysical Research Communications, 486(2), 378-384. https://doi.org/10.1016/j.bbrc.2017.03.049