Epithelial cell IκB-kinase β has an important protective role in Clostridium difficile toxin A-induced mucosal injury

Sungwon Chae, Lars Eckmann, Yukiko Miyamoto, Charalabos Pothoulakis, Michael Karin, Martin F. Kagnoff

    Research output: Contribution to journalArticlepeer-review

    40 Citations (Scopus)


    Toxin A released by Clostridium difficile interacts with the single layer of intestinal epithelial cells that lines the host's intestinal tract and leads to mucosal damage and inflammation that manifests clinically as antibiotic-associated diarrhea and pseudomembranous colitis. Activation of the transcription factor NF-κB in intestinal epithelial cells is important for regulating the expression of epithelial cell proinflammatory genes and cell survival. However, the role of NF-κB activation in the pathogenesis of C. difficile toxin A-induced colitis is unknown. To determine the functional importance in vivo of NF-κB activation in intestinal epithelium in the pathogenesis of C. difficile-induced colitis, we used mutant mice that do not activate the classical NF-κB signaling pathway in intestinal epithelial cells due to a conditional deficiency in those cells of the IκB-kinase β (IKKβ) subunit of IKK. C. difficile toxin A challenge of intestinal loops in intestinal epithelial cell IKKβ-deficient mice induced a rapid and significant increase in intestinal epithelial apoptosis compared with littermate controls. This was accompanied by a significant increase in acute mucosal inflammation, mucosal injury, luminal fluid secretion, and bacterial translocation. We conclude that activation of intestinal epithelial cell NF-κB by toxin A plays an important host mucosal protective role after C. difficile toxin A exposure that is mediated, at least in part, through promoting epithelial cell survival by abrogating epithelial cell apoptosis.

    Original languageEnglish
    Pages (from-to)1214-1220
    Number of pages7
    JournalJournal of Immunology
    Issue number2
    Publication statusPublished - 2006 Jul 15

    ASJC Scopus subject areas

    • Immunology and Allergy
    • Immunology


    Dive into the research topics of 'Epithelial cell IκB-kinase β has an important protective role in Clostridium difficile toxin A-induced mucosal injury'. Together they form a unique fingerprint.

    Cite this