Epithin, a target of transforming growth factor-β signaling, mediates epithelial-mesenchymal transition

Hyo Seon Lee, Chungho Kim, Sang Bum Kim, Moon Gyo Kim, Dongeun Park

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The epithelial-derived type II transmembrane serine protease epithin has been shown to be upregulated in a variety of cancer cell lines and tumor tissues, and its upregulation correlates well with tumor progression in many cases. However, little is known regarding the regulation of its expression and the mechanism of its roles in tumor progression. Here, we show that transforming growth factor-β (TGF-β), a potent inducer of epithelial-mesenchymal transition (EMT) in tumor progression, upregulates epithin, and that epithin plays a critical role in TGF-β-induced EMT. Forced overexpression of epithin induced EMT to exhibit characteristic morphological changes, alternations in EMT-related proteins and enhanced cell motility. Conversely, shRNA-mediated knockdown of endogenous epithin inhibited TGF-β-induced expression of mesenchymal markers and morphological changes. Furthermore, TGF-β-induced cell migration and invasion were significantly impaired by epithin knockdown. In addition, we demonstrate that TGF-β upregulates epithin transcriptionally via the Smad2/Smad4-mediated pathway. These results suggest that epithin is a key mediator of TGF-β-induced EMT in tumor progression.

Original languageEnglish
Pages (from-to)553-559
Number of pages7
JournalBiochemical and biophysical research communications
Volume395
Issue number4
DOIs
Publication statusPublished - 2010 May 14
Externally publishedYes

Keywords

  • Epithelial-mesenchymal transition
  • Epithin
  • TGF-β

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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